Team:Evry/Notebook/Sensing/PCBs/week6

From 2014.igem.org

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<h1> Sensor construction hbpR-hbpC </h1>  
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<u> <h3> Sensor construction hbpR-hbpC </h3> </u>
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<u>Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
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<h4> 08/6/14 :</h4>
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<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
<br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
<br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
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<h4> 08/7/14 :</h4>
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<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
<br/> Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
<br/> Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
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<h4> 08/8/14 :</h4>
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<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
 +
<br/> Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL
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Revision as of 11:34, 19 August 2014

Week 6

Sensor construction hbpR-hbpC

08/6/14 :

Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C

08/7/14 :

Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm

08/8/14 :

Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL

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