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Team:Evry/Notebook/Sensing/PCBs/week6
From 2014.igem.org
(Difference between revisions)
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<div class="week6"> | <div class="week6"> | ||
| - | <h4>Week 6 | + | <h4>Week 6</h4> |
<div class="Sensing/PCBs"> | <div class="Sensing/PCBs"> | ||
<p> | <p> | ||
| - | < | + | <h1> Sensor construction hbpR-hbpC </h1> |
<u>Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | <u>Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | ||
<br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C | <br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C | ||
Revision as of 16:44, 18 August 2014
Week 6
Sensor construction hbpR-hbpC
Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
