Team:ITESM-Guadalajara
From 2014.igem.org
(Prototype team page) |
|||
Line 15: | Line 15: | ||
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p> | <br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p> | ||
<br> | <br> | ||
+ | <p>Chitosan is a polysaccharide found in abundance in shrimp shell. The current extraction methods involve the use of strong acids and alkalis, these substances are dangerous wastes that can compromise the integrity of soil and water where they are disposed.</p> | ||
+ | |||
+ | <p>Due to its stability and biocompatibility, chitosan has been widely used in pharmaceutical, food, and cosmetic industries, among many others. The Chitosan global market has an estimated value of $20 billion USD. Particularly, the pharmaceutical industry represents on average 1.2% of total GDP in Mexico and has grown at a 10% rate since 2008.</p> | ||
+ | |||
+ | <p>Therefore, it is imperative to develop a new and efficient extraction process, that is also eco-friendly. We’ve developed a new process that consists in the pretreatment of shrimp shells, followed by a fermentation in which it is demineralized and deproteinized by B. subtilis. Finally, it goes through a deacetylation process in a fermentation performed by our genetically modified E. coli strain. This modified bacterium contains 6 different enzymes that are found naturally in microorganisms that are capable of cutting and deacetylating chitin for the generation of chitosan. The action of these enzymes and a careful temperature treatment will allow us to generate chitosan with a 90% deacetylation degree. This deacetylation degree is the required one by the pharmaceutical industry, our target market.</p> | ||
+ | |||
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:ITESM-Guadalajara&action=edit"style="color:#FFFFFF"> Click here to edit this page!</a> </p> | <p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:ITESM-Guadalajara&action=edit"style="color:#FFFFFF"> Click here to edit this page!</a> </p> | ||
</td> | </td> |
Revision as of 16:16, 14 August 2014
WELCOME TO iGEM 2014!Your team has been approved and you are ready to start the iGEM season!
Chitosan is a polysaccharide found in abundance in shrimp shell. The current extraction methods involve the use of strong acids and alkalis, these substances are dangerous wastes that can compromise the integrity of soil and water where they are disposed. Due to its stability and biocompatibility, chitosan has been widely used in pharmaceutical, food, and cosmetic industries, among many others. The Chitosan global market has an estimated value of $20 billion USD. Particularly, the pharmaceutical industry represents on average 1.2% of total GDP in Mexico and has grown at a 10% rate since 2008. Therefore, it is imperative to develop a new and efficient extraction process, that is also eco-friendly. We’ve developed a new process that consists in the pretreatment of shrimp shells, followed by a fermentation in which it is demineralized and deproteinized by B. subtilis. Finally, it goes through a deacetylation process in a fermentation performed by our genetically modified E. coli strain. This modified bacterium contains 6 different enzymes that are found naturally in microorganisms that are capable of cutting and deacetylating chitin for the generation of chitosan. The action of these enzymes and a careful temperature treatment will allow us to generate chitosan with a 90% deacetylation degree. This deacetylation degree is the required one by the pharmaceutical industry, our target market. |
||||||||||||
| ||||||||||||
Requirements | ||||||||||||
Please be sure to keep these links, your audience will want to find your: |
There are a few wiki requirements teams must follow:
Visit the Wiki How To page for a complete list of requirements, tips and other useful information. |
|||||||||||
Tips | ||||||||||||
We are currently working on providing teams with some easy to use design templates.
For a full wiki list, you can visit iGEM 2013 web sites and iGEM 2012 web sites lists. |
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
|