Team:Caltech/week8
From 2014.igem.org
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<b>Export Systems</b> | <b>Export Systems</b> | ||
- | <ul> | + | <br>LCMS pTG004-noFLAG (lam system in its native configuration without the 3xFLAG) preparation: |
+ | <ul><li>Miniprepped the liquid culture of pTG004-noFLAG incubated last night. However, the final DNA conc. was too low to send for sequenceing, so the liquid culture was grown up again for another try tomorrow.</li> | ||
+ | </ul> | ||
+ | Western blot negative control preparation: | ||
+ | <ul><li>Ran colony PCR on colonies transformed with A2 (pTet_GFP from Anton).</li> | ||
+ | <li>Set up liquid cultures with carbenicillin for picked colonies.</li> | ||
</ul> | </ul> | ||
<b>lamBCDA & fsrABC Reception Systems</b> | <b>lamBCDA & fsrABC Reception Systems</b> |
Revision as of 23:52, 9 August 2014
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