Team:RHIT/Sustainability

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<h3 style="font-size:18px">Protocols</h3>
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        <label for="checkbox-1">Polymer Degradation</label>
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            <p><p>To a 1 L Erlenmeyer flask add:<p/>
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              <ul>
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              <li>5.0 g shredded PLA</li>
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              <li>5.6 g NaOH pellets</li>
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              <li>50 mL DI water</li>
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              <li>Stir bar</li>
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              <p>Stir reaction, heating to 50-60&#176;C, overnight or until all plastic has visibly dissolved.</p>
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              <br>
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              <p>Neutralize with HCl to a pH of 5-6.</p>
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              <p>Vacuum filter solution 2-3 times, dispose of any solid collected.</p>
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              <p>Using a sterile syringe, filter solution into a jar to store until needed.</p></p>
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        <label for="checkbox-2">Characterization</label>
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            <p><p>The filtered solution was measured in a polarimer. The optical rotation results are as follows.</p>
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              <th style="border:1px solid black">pH</th>
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              <th style="border:1px solid black">Optical Rotation</th>
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              <td style="border:1px solid black">2.5</td>
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              <td style="border:1px solid black">.12</td>
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              <tr style="background-color:#D8D8D8; border:1px solid black">
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              <td style="border:1px solid black">3.5</td>
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              <td style="border:1px solid black">-.13</td>
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              <tr style="background-color:#D8D8D8; border:1px solid black">
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              <td style="border:1px solid black">4.21</td>
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              <td style="border:1px solid black">-.26</td>
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              </tr>
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              </table>
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              <p>This suggests that the solution did in fact contain lactate because a pH change made a significant difference in the optical rotation of the solution.</p></p>
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        <label for="checkbox-3">LDH Assay</label>
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Revision as of 20:08, 6 August 2014

PLA Recycling


Protocols

  • To a 1 L Erlenmeyer flask add:

    • 5.0 g shredded PLA
    • 5.6 g NaOH pellets
    • 50 mL DI water
    • Stir bar

    Stir reaction, heating to 50-60°C, overnight or until all plastic has visibly dissolved.


    Neutralize with HCl to a pH of 5-6.

    Vacuum filter solution 2-3 times, dispose of any solid collected.

    Using a sterile syringe, filter solution into a jar to store until needed.

  • The filtered solution was measured in a polarimer. The optical rotation results are as follows.

    pH Optical Rotation
    2.5 .12
    3.5 -.13
    4.21 -.26

    This suggests that the solution did in fact contain lactate because a pH change made a significant difference in the optical rotation of the solution.

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