Team:Duke/Notebook/MayJun
From 2014.igem.org
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+ | <td colspan="7"> May 2014 </td> | ||
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+ | <td colspan="7" align=center><i> Month 2 of Project </i></td> | ||
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+ | <td>26</td> | ||
+ | <td>27</td> | ||
+ | <td>28</td> | ||
+ | <td><a href="https://2014.igem.org/Team:Duke/Notebook#may29">29</a></td> | ||
+ | <td>30</td> | ||
+ | <td>31</td> | ||
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Revision as of 19:03, 29 July 2014
May 2014 | ||||||
Month 2 of Project | ||||||
sun | mon | tue | wed | thu | fri | sat |
1 | 2 | 3 | ||||
4 | 5 | 6 | 7 | 8 | 9 | 10 |
11 | 12 | 13 | 14 | 15 | 16 | 17 |
18 | 19 | 20 | 21 | 22 | 23 | 24 |
25 | 26 | 27 | 28 | 29 | 30 | 31 |
May 29
Objective: Prepare Chemically competent E. Coli
Matthew Faw
Followed Charlie’s Cloning Protocols to prepare chemically competent E. Coli
- Once E. Coli were prepared, the solution was aliquoted into 12 labeled tubes (450l in each tube) and tubes were stored in iGEM box in -80C cooler on far left
Next Steps:
- Transformation
June 1
Objective: Transform Chemically competent E. Coli (CCEC)
Matthew Faw
Transformation of CCEC with RFP Construct of varying concentrations+control with no DNA
- Followed Charlie’s Cloning Protocols
- Mistakenly used all of DNA construct from the kit… So not sure if the transformation will be successful
- Plated the transformed DNA and put in incubator at 37C
- Results (6/2/14)--Transformation unsuccessful because improper procedure was done by MFaw
Next Steps:
- Look at plates, compare cultures
June 2
Objective: Redo yesterday’s transformation
Matthew Faw
Due to my failure to correctly conduct the transformation yesterday, it was necessary to redo the transformation.
- Followed Charlie’s Cloning Protocols
- Added 1 ul of .5, 5, 10, 20, 50, and 0 (control) pg/lof RFP Construct to chemically competent E. Coli (CCEC), and followed procedure
- Plated the transformed DNA on 6 separate plates, put in 37C overnight
- Results: (6/3/14)-Transformation unsuccessful. Try the transformation again with Charlie’s cells and with new cells grown using iGEM’s protocol
Next Steps:
- Look at plates, compare cultures, etc.