Team:Caltech/Project/Methods and Methods
From 2014.igem.org
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<ul><li> Add 2 μL of plasmid to 20 μL of cells</li> | <ul><li> Add 2 μL of plasmid to 20 μL of cells</li> | ||
<li> Stir with pipette tip</li> | <li> Stir with pipette tip</li> | ||
- | <li> Keep cells on ice</li> | + | <li> Keep cells on ice for 5-10 minutes</li> |
+ | <li> (Optional if plating onto CARB) Add 4 volumes of SOC medium and incubate in shaker at 37°C for 1 hour</li> | ||
<li> Plate with glass beads </li> | <li> Plate with glass beads </li> | ||
</ul> | </ul> |
Revision as of 18:25, 24 July 2014
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Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
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