Team:Caltech/week6

From 2014.igem.org

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     <li>Assemblies were transformed into JM109, plated on carbenicillin plates, and incubated overnight</li>
     <li>Assemblies were transformed into JM109, plated on carbenicillin plates, and incubated overnight</li>
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<b>lamBCDA & fsrABC Reception Systems</b>
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<b>agrBCDA Reception System and Combinatorial Promoters</b>
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For the agrBCDA System, we troubleshot our problems creating the pAA009/10 vector backbone:
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<ul><li>Ran a gel of the PCR product created on Friday (pAA009/10 backbone). Results were negative.</li>
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    <li>Tried PCR again with lower annealing temperatures [(what temps?)]. Results were negative again.</li>
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</ul>
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For the combinatorial promoters:
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<ul><li>Set up overnight culture of jwAA001 strain to do more experiments tomorrow</li></ul>
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Revision as of 06:17, 22 July 2014
Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions
Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week Six

Monday, 7/21/14

 Export Systems 3 of our 5 constructs were not properly cloned, so we are going to redo them:
  • PCRed out backbones for pTG002, pTG003, and pTG006 with overhangs
  • PCR product was then DpnI digested and PCR-purified. A gel was run with processed and unprocessed product to verify backbone creation
  • Gibson assembly of pTG002, pTG003, and pTG006 (pTG006 had 2 reactions: 1 with the backbone created last week, since it had been verified to work for cloning pTG005, and another with backbone created today)
  • Assemblies were transformed into JM109, plated on carbenicillin plates, and incubated overnight
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters For the agrBCDA System, we troubleshot our problems creating the pAA009/10 vector backbone:
  • Ran a gel of the PCR product created on Friday (pAA009/10 backbone). Results were negative.
  • Tried PCR again with lower annealing temperatures [(what temps?)]. Results were negative again.
For the combinatorial promoters:
  • Set up overnight culture of jwAA001 strain to do more experiments tomorrow

Tuesday, 7/22/14

Wednesday, 7/23/14

Thursday, 7/24/14

Friday, 7/25/14

Retrieved from "http://2014.igem.org/Team:Caltech/week6"