Team:Paris Saclay/Notebook/July/11

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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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===3 - Bacterial Culture===
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''by Mathieu''
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Strains used: J23106 and J23114 from the concentrated colony made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July]
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5uL of antibiotic in each tube.
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 15:40, 21 July 2014

Contents

Friday 11th July

Lab work

1 - Fill in the WIKI (Notebook and Protocols)

by Juliette & Romain

2 - Preparation of competent cells E.coli

by Maher and Sean

Competent strains used:

  • DH5α
  • MG1655Z1

Sean did the first day, Maher did the second one.

Protocol

3 - Electrophoresis

by Fabio & Marie

Electrophoresis 11.07.2014.jpg
  1. Cm FNR-RBS-AmilCP term (1)
  2. Cm FNR-RBS-AmilCP term (2)
  3. B0015 (1)
  4. B0015 (2)
  5. PCola-GES 10^-2 (1)
  6. PCola-GES 10^-2 (1)
  7. PCola-GES 10^-2 (2)
  8. PCola-GES 10^-2 (2)
  9. PJBG-6409 10^-2 (1)
  10. PJBG-6409 10^-2 (2)
  11. PJBG-6409 10^-2 (2)

Protocol

3 - Bacterial Culture

by Mathieu

Strains used: J23106 and J23114 from the concentrated colony made the 10th July

5uL of antibiotic in each tube.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/11"