Team:Jilin China/Blueprint

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<h2>Result</h2>
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<h2>Application future</h2>
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<p>Mlr A has significant degradation function to MC-LR, and at certain degree of MC-LR, the natural promoter will promote the expression of gene in engineering bacteria. This is a lucky accident. Before this, we have tried to use PP2A to monitor the concentration of MC-LR. But we found it was really hard for us to do the gene control in Prokaryotic cells. However, we are lucky to find the natural promoter of Mlr A which gave us a new approach to realize the goal of our project.</p>
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<p>Microcystin-LR very stable and in some extent is very resistant to high temperature and pH change, so it is difficult to be clear out by an ordinary drinking water treatment process. After a large-scale outbreak of blue-green algae, microcystin-LR presence can be detected in drinking water in residential home. Because the great damage to liver, microcystin-LR must be removed form the residents drinking water.</p>
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<h2>Blueprint</h2>
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<p>There are a variety of microcystin biodegradation reports, in the degradation pathway, microcystinase (MlrA) is the first enzyme to hydrolyze cyclic microcystin LR into a linear intermediate. Because the toxicity of linear microcystin LR decreases about 160 times, MlrA has been regarded as a crucial enzyme for removal of the toxin (Bourne et al., 1996).</p>
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<p>Until I write this paragraph, our team have found a promoter to detect the toxin and a enzyme to decompose it. The result show a good biological activity and I think it can be used in our daily life what I will discuss next. We can see that we already achieve our goal：detecting the density of MC-LR and release MlrA by itself. Our team call it “Automatization machine control”.</p>
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<p>Mlr promoter is able to detect whether water containing microcystin LR and start to express the GFP-mlrA fusion protein. GFP can emit green fluorescence, very simple and very sensitive to tell us the water contains toxic microcystin LR. MlrA can directly degrade microcystin LR and eliminate its toxicity, eliminate the toxins in water pollution.</p>
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<p>We can use this result in water cleansing by clearing MC-LR before people drink or use. According to report, MC-LR can destroy human’s liver, so what we do not only protect environment but make people healthier. Instead of E.coli, we use lactic acid bacteria as our engineering bacteria to obey the rule that working for people’s healthy.</p>
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<p>The mlr promoter-GFP-mlrA was be cloned into the E. coli-L. lactis shuttle expression vector pMG36e, so it can be able to express in E. coli and Lactococcus lactis, which Can easily be constructed in the laboratory, at the same time to avoid secondary pollution in the natural environment of E. coli.</p>
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<p>Lower the danger level and make it more automate, we got it and will make it much better.</p>
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Latest revision as of 03:57, 18 October 2014

Welcome!
Team Jilin_China

Application future

Microcystin-LR very stable and in some extent is very resistant to high temperature and pH change, so it is difficult to be clear out by an ordinary drinking water treatment process. After a large-scale outbreak of blue-green algae, microcystin-LR presence can be detected in drinking water in residential home. Because the great damage to liver, microcystin-LR must be removed form the residents drinking water.

There are a variety of microcystin biodegradation reports, in the degradation pathway, microcystinase (MlrA) is the first enzyme to hydrolyze cyclic microcystin LR into a linear intermediate. Because the toxicity of linear microcystin LR decreases about 160 times, MlrA has been regarded as a crucial enzyme for removal of the toxin (Bourne et al., 1996).

Mlr promoter is able to detect whether water containing microcystin LR and start to express the GFP-mlrA fusion protein. GFP can emit green fluorescence, very simple and very sensitive to tell us the water contains toxic microcystin LR. MlrA can directly degrade microcystin LR and eliminate its toxicity, eliminate the toxins in water pollution.

The mlr promoter-GFP-mlrA was be cloned into the E. coli-L. lactis shuttle expression vector pMG36e, so it can be able to express in E. coli and Lactococcus lactis, which Can easily be constructed in the laboratory, at the same time to avoid secondary pollution in the natural environment of E. coli.

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