Team:HokkaidoU Japan/Notebook/asB0034/asB0034-construction

(Difference between revisions)

Revision as of 03:50, 18 October 2014

Notebook

Lab Documents

Start
Primer Annealing
- Sun Aug 17
asB0034_F Tm:68.7℃ asB0034_R Tm:68.7℃
Digestion
- Sun Aug 17
Cut pHN1257 with NcoI, XhoI(using 10×cut smart)
Gel extraction
- Mon Aug 18
digested pHN1257
Ethanol Precipetation
- Mon Aug 18
digested pHN1257
Ligation
- Mon Aug 18
Ligate pHN1257 with asB0034
Transformation
- Tue Aug 19
Ptrc-stem-asB0034-stem on pHN1257

5.0µL DNA to DH5α
Colony PCR
- Wed Aug 20
Ptrc-stem-asB0034-stem(on pHN1257) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃
Culture
- Thu Aug 21
Ptrc-stem-asB0034-stem(on pHN1257)
Mini-Prep
- Fri Aug 22
Ptrc-stem-asB0034-stem(on pHN1257)
Double Transformation
- Fri Aug 22
Ptrc-stem-asB0034-stem(on pHN1257)&R0040-B0034-E1010-B0015(on pSB6A1)

each of 2.5µL DNA to DH5α
Complete!

@@ Line 243: / Line 243: @@
 	      </ul>
 	      <div class="step-recipe">
-	Ptrc-stem-asB0034-stem on pHN1257
+Ptrc-stem-asB0034-stem on pHN1257
-.0&micro;L DNA to DH5&alpha;
+</div>
+<div class="step-recipe">
+.0&micro;L DNA to DH5&alpha;
 	    </div>
@@ Line 294: / Line 296: @@
 	  <div class="step-tile">
 	  <h2 class="step-title">
-mini-Prep
+Mini-Prep
 </h2>
 	  </div>
@@ Line 329: / Line 331: @@
 	      <div class="step-recipe">
 	Ptrc-stem-asB0034-stem(on pHN1257)&R0040-B0034-E1010-B0015(on pSB6A1)
+</div>
+<div class="step-recipe">
                                        each of 2.5&micro;L DNA to DH5&alpha;
 </div>