Team:HokkaidoU Japan/Notebook/asB0034/Ptet B0034 32 asB0034 assay

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Revision as of 03:20, 18 October 2014

Notebook
Lab Documents

Ptet-B0034,B0032,-mRFP-dT
Ptet-B0032-mRFP-dT&asB0034Assay

  • Start

  • Preculture

    R0040-B0034-E1010-B0015 (on pSB6A1) R0040-B0032-E1010-B0015 (on pSB6A1) Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0032-E1010-B0015 (on pSB6A1) Turbidity was controlled 0.1

  • Culture

    M9ZB 2 mL IPTG 20 µ or not for 9 hours, 11 hours, 13hours

  • Assay

    fluoresence imageing 514 nm 610 filter

  • Complete!

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