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Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 mRFP Assay
From 2014.igem.org
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| - | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) each of 2.5µL to DH5&alph | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) each of 2.5µL to DH5&alph; |
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| - | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034_R Tm:68.7℃ |
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Revision as of 02:12, 18 October 2014
Notebook
Lab Documents
140910 asB0034&B0034 mRFP Assay
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Start
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Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) each of 2.5µL to DH5&alph; -
Colony PCR
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034_R Tm:68.7℃ -
Preculture
Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) LB 2 mL for 3hours Turbidity was controlled 0.1 -
Culture
LB 2 mL IPTG 20 µ or not for 11 hours, 13hours, 15hours, 17 hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
