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Team:Bordeaux/NotebookAugust18
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Revision as of 01:42, 18 October 2014
4th June
Molecular biology
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Chewback: linearization of ELP-40
ELP-40 miniprep product 1μl
Primer ELP-40 forward 2,5μl
Primer ELP-40 reverse 2,5μl
dNTP (10mM) 1μl
enzyme 0,5μl
Buffer 5X 10μl
H2O qsp 50μl
PCR program
3min 95°C
25 cycles :
30sec 95°C5min 72°C
30sec 45°C
3min 72°C
Migration on agarose 1% gel (30min, 100V)
Production
Preculture of ELP-60 in 500ml TB medium
