From 2014.igem.org

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18^th August

• Chewback: linearization of ELP-40
  ELP-40 miniprep product 1μl
  Primer ELP-40 forward 2,5μl
  Primer ELP-40 reverse 2,5μl
  dNTP (10mM) 1μl
  enzyme 0,5μl
  Buffer 5X 10μl
  H2O qsp 50μl
  
  PCR program
  3min 95°C
  25 cycles :
  
  30sec 95°C
  30sec 45°C
  3min 72°C
  
  5min 72°C
  
  Migration on agarose 1% gel (30min, 100V)