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Team:Cornell/notebook/plasmids
From 2014.igem.org
(Difference between revisions)
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<tr><td>pA14G</td><td>invalid</td><td>A-Promoter+RBS+merT+merP</td><td>A+F</td><td>pJ61002</td><td>Ampicillin</td></tr> | <tr><td>pA14G</td><td>invalid</td><td>A-Promoter+RBS+merT+merP</td><td>A+F</td><td>pJ61002</td><td>Ampicillin</td></tr> | ||
<tr><td>pC14H</td><td>BBa_J04450</td><td>mRFP in pSB1C3</td><td>pC13F</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | <tr><td>pC14H</td><td>BBa_J04450</td><td>mRFP in pSB1C3</td><td>pC13F</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | ||
| - | <tr><td>pC14I</td><td> | + | <tr><td>pC14I</td><td>not submitted</td><td>A-Promoter+RBS+merT+merP+ter BioBrick</td><td>AG+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pA14J</td><td>invalid</td><td>A-Promoter+RBS+nixA</td><td>C+F</td><td>pJ61002</td><td>Ampicillin</td></tr> | <tr><td>pA14J</td><td>invalid</td><td>A-Promoter+RBS+nixA</td><td>C+F</td><td>pJ61002</td><td>Ampicillin</td></tr> | ||
| - | <tr><td>pC14K</td><td> | + | <tr><td>pC14K</td><td>not submitted</td><td>A-Promoter+RBS+nixA+ter BioBrick</td><td>AF+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pAK14L</td><td>invalid</td><td>T7+GST-YMT</td><td>B+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr> | <tr><td>pAK14L</td><td>invalid</td><td>T7+GST-YMT</td><td>B+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr> | ||
| - | <tr><td>pC14M</td><td> | + | <tr><td>pC14M</td><td>not submitted</td><td>T7+GST-YMT+ter in pSB1C3</td><td>L+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pAK14N</td><td>invalid</td><td>T7+GST-PMT</td><td>D+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr> | <tr><td>pAK14N</td><td>invalid</td><td>T7+GST-PMT</td><td>D+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr> | ||
| - | <tr><td>pC14O</td><td> | + | <tr><td>pC14O</td><td>not submitted</td><td>T7+GST-PMT+ter</td><td>N+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14P</td><td> | + | <tr><td>pC14P</td><td>not submitted</td><td>T7+GST-YMT BioBrick</td><td>M</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14Q</td><td> | + | <tr><td>pC14Q</td><td>not submitted</td><td>T7+GST-PMT BioBrick</td><td>O</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pA14R</td><td>invalid</td><td>A-Promoter+RBS+ CBP4 + Biobrick prefix and suffix</td><td>BioBasic</td><td>pUC57</td><td>Ampicillin</td></tr> | <tr><td>pA14R</td><td>invalid</td><td>A-Promoter+RBS+ CBP4 + Biobrick prefix and suffix</td><td>BioBasic</td><td>pUC57</td><td>Ampicillin</td></tr> | ||
| - | <tr><td>pC14S</td><td> | + | <tr><td>pC14S</td><td>not submitted</td><td>A-promoter+RBS+CBP4 BioBrick</td><td>R+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pC14T</td><td>BBa_B1006</td><td>Terminator</td><td>2013 KP: 6D</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | <tr><td>pC14T</td><td>BBa_B1006</td><td>Terminator</td><td>2013 KP: 6D</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | ||
| - | <tr><td>pC14U</td><td> | + | <tr><td>pC14U</td><td>not submitted</td><td>Final mercury + GST-YMT construct</td><td>I+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14V</td><td> | + | <tr><td>pC14V</td><td>not submitted</td><td>Final nickel + GST-YMT construct</td><td>K+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14W</td><td> | + | <tr><td>pC14W</td><td>not submitted</td><td>Final lead + GST-YMT construct</td><td>S+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14X</td><td> | + | <tr><td>pC14X</td><td>not submitted</td><td>Final mercury + GST-PMT construct</td><td>I+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14Y</td><td> | + | <tr><td>pC14Y</td><td>not submitted</td><td>Final nickel + GST-PMT construct</td><td>K+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14Z</td><td> | + | <tr><td>pC14Z</td><td>not submitted</td><td>Final lead + GST-PMT construct</td><td>S+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pC14AA</td><td>BBa_K540001</td><td>Nickel inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | <tr><td>pC14AA</td><td>BBa_K540001</td><td>Nickel inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | ||
<tr><td>pC14AB</td><td>BBa_K346002</td><td>mercury inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | <tr><td>pC14AB</td><td>BBa_K346002</td><td>mercury inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | ||
<tr><td>pC14AC</td><td>BBa_K206000</td><td>pBAD</td><td>2014 KP3: 14A</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | <tr><td>pC14AC</td><td>BBa_K206000</td><td>pBAD</td><td>2014 KP3: 14A</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | ||
| - | <tr><td>pC14AD</td><td> | + | <tr><td>pC14AD</td><td>not submitted</td><td>Nickel inducible + mRFP</td><td>AA+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
| - | <tr><td>pC14AE</td><td> | + | <tr><td>pC14AE</td><td>not submitted</td><td>Mercury inducible + mRFP</td><td>AB+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pA14AF</td><td>invalid</td><td>NixA Synthesized (A-promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr> | <tr><td>pA14AF</td><td>invalid</td><td>NixA Synthesized (A-promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr> | ||
<tr><td>pA14AG</td><td>invalid</td><td>MerT/MerP Synthesized (A-Promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr> | <tr><td>pA14AG</td><td>invalid</td><td>MerT/MerP Synthesized (A-Promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr> | ||
<tr><td>pA14AH</td><td>invalid</td><td>GST-YMT Synthesized (pT7, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr> | <tr><td>pA14AH</td><td>invalid</td><td>GST-YMT Synthesized (pT7, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr> | ||
| - | <tr><td>pC14AI</td><td> | + | <tr><td>pC14AI</td><td>not submitted</td><td>GST-YMT Synthesized (pT7, RBS, ter) in pSB1C3</td><td>AH+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
<tr><td>pC14AJ</td><td>BBa_K592009</td><td>amilCP in pSB1C3</td><td>2014 KP1: 19E</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | <tr><td>pC14AJ</td><td>BBa_K592009</td><td>amilCP in pSB1C3</td><td>2014 KP1: 19E</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> | ||
| - | <tr><td>pC14AK</td><td> | + | <tr><td>pC14AK</td><td>not submitted</td><td>GST-YMT Synthesized without T7 (RBS,ter) in pSB1C3</td><td>AI + T7</td><td>pSB1C3</td><td>Chloramphenicol</td></tr> |
</table> | </table> | ||
</div> | </div> | ||
Latest revision as of 01:41, 18 October 2014
Plasmids
| Plasmid Name | Part | Contents/Description | Origin | Backbone | Antibiotic Resistance |
|---|---|---|---|---|---|
| pA14A | pDH1 | mer operon | Wilson | pCL1921 | Ampicillin |
| pA14B | pGYMT | GST-YMT | Wilson | pGEX-2T | Ampicillin |
| pA14C | pUEF202 | nixA | Wilson | pBluescript | Ampicillin |
| pA14D | pGPMT | GST-PMT | Wilson | pGPMT | Ampicillin |
| pAK14E | BBa_I712074 | T7 promoter | pAK13D | pSB1AK8 | Ampicillin |
| pA14F | BBa_J23102 | Anderson Promoter+mRFP | 2013 KP5: 18G | pJ61002 | Ampicillin |
| pA14G | invalid | A-Promoter+RBS+merT+merP | A+F | pJ61002 | Ampicillin |
| pC14H | BBa_J04450 | mRFP in pSB1C3 | pC13F | pSB1C3 | Chloramphenicol |
| pC14I | not submitted | A-Promoter+RBS+merT+merP+ter BioBrick | AG+H | pSB1C3 | Chloramphenicol |
| pA14J | invalid | A-Promoter+RBS+nixA | C+F | pJ61002 | Ampicillin |
| pC14K | not submitted | A-Promoter+RBS+nixA+ter BioBrick | AF+T | pSB1C3 | Chloramphenicol |
| pAK14L | invalid | T7+GST-YMT | B+E | pSB1AK8 | Ampicillin |
| pC14M | not submitted | T7+GST-YMT+ter in pSB1C3 | L+T | pSB1C3 | Chloramphenicol |
| pAK14N | invalid | T7+GST-PMT | D+E | pSB1AK8 | Ampicillin |
| pC14O | not submitted | T7+GST-PMT+ter | N+T | pSB1C3 | Chloramphenicol |
| pC14P | not submitted | T7+GST-YMT BioBrick | M | pSB1C3 | Chloramphenicol |
| pC14Q | not submitted | T7+GST-PMT BioBrick | O | pSB1C3 | Chloramphenicol |
| pA14R | invalid | A-Promoter+RBS+ CBP4 + Biobrick prefix and suffix | BioBasic | pUC57 | Ampicillin |
| pC14S | not submitted | A-promoter+RBS+CBP4 BioBrick | R+H | pSB1C3 | Chloramphenicol |
| pC14T | BBa_B1006 | Terminator | 2013 KP: 6D | pSB1C3 | Chloramphenicol |
| pC14U | not submitted | Final mercury + GST-YMT construct | I+P | pSB1C3 | Chloramphenicol |
| pC14V | not submitted | Final nickel + GST-YMT construct | K+P | pSB1C3 | Chloramphenicol |
| pC14W | not submitted | Final lead + GST-YMT construct | S+P | pSB1C3 | Chloramphenicol |
| pC14X | not submitted | Final mercury + GST-PMT construct | I+Q | pSB1C3 | Chloramphenicol |
| pC14Y | not submitted | Final nickel + GST-PMT construct | K+Q | pSB1C3 | Chloramphenicol |
| pC14Z | not submitted | Final lead + GST-PMT construct | S+Q | pSB1C3 | Chloramphenicol |
| pC14AA | BBa_K540001 | Nickel inducible | Kit Plate | pSB1C3 | Chloramphenicol |
| pC14AB | BBa_K346002 | mercury inducible | Kit Plate | pSB1C3 | Chloramphenicol |
| pC14AC | BBa_K206000 | pBAD | 2014 KP3: 14A | pSB1C3 | Chloramphenicol |
| pC14AD | not submitted | Nickel inducible + mRFP | AA+H | pSB1C3 | Chloramphenicol |
| pC14AE | not submitted | Mercury inducible + mRFP | AB+H | pSB1C3 | Chloramphenicol |
| pA14AF | invalid | NixA Synthesized (A-promoter, RBS, ter) | GenScript | pUC57 | Ampicillin |
| pA14AG | invalid | MerT/MerP Synthesized (A-Promoter, RBS, ter) | GenScript | pUC57 | Ampicillin |
| pA14AH | invalid | GST-YMT Synthesized (pT7, RBS, ter) | GenScript | pUC57 | Ampicillin |
| pC14AI | not submitted | GST-YMT Synthesized (pT7, RBS, ter) in pSB1C3 | AH+H | pSB1C3 | Chloramphenicol |
| pC14AJ | BBa_K592009 | amilCP in pSB1C3 | 2014 KP1: 19E | pSB1C3 | Chloramphenicol |
| pC14AK | not submitted | GST-YMT Synthesized without T7 (RBS,ter) in pSB1C3 | AI + T7 | pSB1C3 | Chloramphenicol |
