Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0032 mRFP Assay

(Difference between revisions)

Revision as of 00:40, 18 October 2014

Notebook

Lab Documents

Start
Transformation
- Sun Sep 7
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0032-E1010-dt(on pSB6A1) each of 2.5µL DNA to DH5α
Colony PCR
- Mon Sep 8
R0040-B0032-E1010-B0015(on pSB6A1) EcoRI_XbaI_F Tm:70.9℃ & RBS_SpeI_R Tm:59.2℃
Preculture
- Tue Sep 9
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0032-E1010-B0015(on pSB6A1) LB 2 mL for 8hours Turbidity was controlled 0.1
Culture
- Tue Sep 9
M9ZB 2 mL IPTG 20 µ or not for 9 hours, 11 hours, 13hours
Assay
- Wed Sep 10
fluoresence imageing 514 nm 610 filter
Complete!

@@ Line 139: / Line 139: @@
 	      </ul>
 	      <div class="step-recipe">
-Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0032-mRFP-dt on pSB6A1	on ice 		 30min each of 2.5&micro;L DNA to DH5&alpha; 42&#8451;    		 30sec on ice  	 2min
+Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0032-E1010-dt(on pSB6A1)	               each of 2.5&micro;L DNA to DH5&alpha;
 </div>
 	    </div>
@@ Line 160: / Line 161: @@
 	      </ul>
 	      <div class="step-recipe">
-Ptet-B0032-mRFP-dT on pSB6A1 EcoI_XbaI_F	Tm:70.9&#8451; rbs_SpeI_R	Tm:59.2&#8451;
+	R0040-B0032-E1010-B0015(on pSB6A1) EcoRI_XbaI_F	Tm:70.9&#8451; & RBS_SpeI_R	Tm:59.2&#8451;
 </div>
 	    </div>
@@ Line 181: / Line 183: @@
 	      </ul>
 	      <div class="step-recipe">
-Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0032-mRFP-dT(pSB6A1) LB 2 mL for 8hours
+Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0032-E1010-B0015(on pSB6A1)          LB 2 mL for 8hours Turbidity was controlled 0.1
 </div>
 	    </div>