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Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 GFP Assay
From 2014.igem.org
(Difference between revisions)
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<div class="step-tile"> | <div class="step-tile"> | ||
<h2 class="step-title"> | <h2 class="step-title"> | ||
| - | Transformation | + | Double Transformation |
</h2> | </h2> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem on pHN1257& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1) |
| - | + | </div> | |
| - | + | <div class="step-reipe"> | |
| + | each of 2.5µL DNA to DH5α | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem(pHN1257)& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃ |
| - | + | ||
| - | + | ||
</div> | </div> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem(pHN1257)& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1) |
| - | + | </div> | |
| - | + | <div class="step-recipe"> | |
| + | LB 2 mL | ||
| + | </div> | ||
| + | <div class="step-recipe"> | ||
| + | for 9hours | ||
| + | </div> | ||
| + | |||
| + | |||
Turbidity was controlled 0.1 | Turbidity was controlled 0.1 | ||
</div> | </div> | ||
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<div class="step-recipe"> | <div class="step-recipe"> | ||
M9ZB 2 mL | M9ZB 2 mL | ||
| + | |||
| + | </div> | ||
IPTG 20 µ or not | IPTG 20 µ or not | ||
| + | </div> | ||
| + | <div class="step-recipe"> | ||
for 9 hours, 11 hours, 13hours | for 9 hours, 11 hours, 13hours | ||
</div> | </div> | ||
Revision as of 00:13, 18 October 2014
Notebook
Lab Documents
140910 asB0034 & B0034 GFP Assay
-
Start
-
Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)each of 2.5µL DNA to DH5α -
Colony PCR
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃ -
Preculture
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)LB 2 mLfor 9hoursTurbidity was controlled 0.1
Culture
M9ZB 2 mL
IPTG 20 µ or not
for 9 hours, 11 hours, 13hours
Assay
fluoresence imageing 488 nm 530 filter
Complete!
