Team:Bordeaux/NotebookJune20

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   <li>Exposure at CuCl2</li>
   <li>Exposure at CuCl2</li>
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=> SDS PAGE
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=> SDS PAGE<br>
Digestion psB6A1 (miniprep 10/06/14) and VPGXG 20 (PCR 10/06/14)
Digestion psB6A1 (miniprep 10/06/14) and VPGXG 20 (PCR 10/06/14)
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</table>
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1h at 37°C + 5µl of digestion -> electrophoresis
1h at 37°C + 5µl of digestion -> electrophoresis
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<br><br>
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<li>ELP <ul class="strain-title"><li>To quantify => scale ELP pellets</li><li>HPLC</li><li>Find a solvant who precipitate ELP immediately.</li></ul>
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<li>ELP <ul class="strain-title"><li>To quantify => scale ELP pellets</li><li>HPLC</li><li>Find a solvant who precipitate ELP immediately.</li></ul><br>
Electrophoresis of PCR of 19/06/14 and VPGXG 20 of 16/06/14</li>
Electrophoresis of PCR of 19/06/14 and VPGXG 20 of 16/06/14</li>
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Latest revision as of 22:40, 17 October 2014

20th June

Molecular biology

  • Preparation of samples :

    • 1st sample :
    • Pellet of 2ml
    • Add SB1X
    • Add beads => vortex
    • 5 min at 85°C
    2nd sample 
    • 2ml of purification product
    • 10µL of sample
    • 10µl of SB 2X
    • Exposure at CuCl2
    => SDS PAGE
    Digestion psB6A1 (miniprep 10/06/14) and VPGXG 20 (PCR 10/06/14)
    Plasmid digestion Insert digestion
    H2O 5,6µl 16,38µl
    10X buffer 4 2µl 2µl
    XbaI 0,25µl 0,25µL
    EcoRI 0,25µl 0,25µl
    DNA 11,9µl 1,12µl
    1h at 37°C + 5µl of digestion -> electrophoresis

  • ELP
    • To quantify => scale ELP pellets
    • HPLC
    • Find a solvant who precipitate ELP immediately.

    Electrophoresis of PCR of 19/06/14 and VPGXG 20 of 16/06/14

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