Team:Caltech/week5
From 2014.igem.org
(Difference between revisions)
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<ul><li>Created glycerol stocks of the overnight liquid cultures (with bacteria transformed with pWW2149+pWW1521 and pWW2149+pWW1523) and stored them at -80°C</li> | <ul><li>Created glycerol stocks of the overnight liquid cultures (with bacteria transformed with pWW2149+pWW1521 and pWW2149+pWW1523) and stored them at -80°C</li> | ||
<li>The remainder of the overnight cultures were then grown in clear MOPS media</li> | <li>The remainder of the overnight cultures were then grown in clear MOPS media</li> | ||
- | <li>Different concentrations of arabinose [specifically what concentrations?] were added separate aliquots of the cultures in a plate | + | <li>Different concentrations of arabinose [specifically what concentrations?] were added to separate aliquots of the cultures in a 96-well plate</li> |
- | <li>GFP fluorescence data was collected from these colonies | + | <li>GFP fluorescence data was collected from these colonies in a plate reader over [how many hours post-induction?]</li> |
</ul></li> | </ul></li> | ||
<li>Construction of lam & fsr Reception Systems | <li>Construction of lam & fsr Reception Systems | ||
<ul><li>PCRed pKS001 template to extract vector backbone with overhangs for later Gibson assembly</li> | <ul><li>PCRed pKS001 template to extract vector backbone with overhangs for later Gibson assembly</li> | ||
</ul></li> | </ul></li> | ||
+ | </ul> | ||
+ | <b>agrBCDA Reception System and Combinatorial Promoters</b> | ||
+ | <ul> | ||
</ul> | </ul> | ||
</td> | </td> |
Revision as of 17:49, 16 July 2014
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