"
Team:OUC-China/Judging
From 2014.igem.org
| Line 207: | Line 207: | ||
<p class="lead">Type: Composite</p> | <p class="lead">Type: Composite</p> | ||
<p class="lead">Length:1448bp</p> | <p class="lead">Length:1448bp</p> | ||
| - | <p class="lead">Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid | + | <p class="lead">Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.</p> |
| - | mobility.</p> | + | |
<p class="lead"></p> | <p class="lead"></p> | ||
<p class="lead">Lysis device induced by L-arabinose</p> | <p class="lead">Lysis device induced by L-arabinose</p> | ||
| Line 227: | Line 226: | ||
<p class="lead">Length: 5141</p> | <p class="lead">Length: 5141</p> | ||
<p class="lead">Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</p> | <p class="lead">Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</p> | ||
| - | |||
| - | |||
<p class="lead">TAT-H4</p> | <p class="lead">TAT-H4</p> | ||
<p class="lead">ID:BBa_K1439004</p> | <p class="lead">ID:BBa_K1439004</p> | ||
| Line 234: | Line 231: | ||
<p class="lead">Length:393</p> | <p class="lead">Length:393</p> | ||
<p class="lead">Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.</p> | <p class="lead">Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.</p> | ||
| - | |||
<p class="lead">TAT-PTD</p> | <p class="lead">TAT-PTD</p> | ||
<p class="lead">ID:BBa_K1439005</p> | <p class="lead">ID:BBa_K1439005</p> | ||
| Line 240: | Line 236: | ||
<p class="lead">Length:33</p> | <p class="lead">Length:33</p> | ||
<p class="lead">Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.</p> | <p class="lead">Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.</p> | ||
| - | |||
<p class="lead">Histone H4</p> | <p class="lead">Histone H4</p> | ||
<p class="lead">ID:BBa_K1439006</p> | <p class="lead">ID:BBa_K1439006</p> | ||
| Line 246: | Line 241: | ||
<p class="lead">Length:312</p> | <p class="lead">Length:312</p> | ||
<p class="lead">Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.</p> | <p class="lead">Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.</p> | ||
| - | |||
<p class="lead">CMV promoter</p> | <p class="lead">CMV promoter</p> | ||
<p class="lead">ID:BBa_K1439007</p> | <p class="lead">ID:BBa_K1439007</p> | ||
| Line 252: | Line 246: | ||
<p class="lead">Length:588</p> | <p class="lead">Length:588</p> | ||
<p class="lead">Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.</p> | <p class="lead">Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.</p> | ||
| - | |||
<p class="lead">TAT-H4-B0015</p> | <p class="lead">TAT-H4-B0015</p> | ||
<p class="lead">ID:BBa_K1439008</p> | <p class="lead">ID:BBa_K1439008</p> | ||
Revision as of 22:00, 17 October 2014
Origin of transfer for the RP4-plasmid nic region
ID: BBa_K1439000
Type: conjugation
Length:350bp
Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.
[OriTRP4]+[RFP]
ID: BBa_K1439001
Type: Composite
Length:1427bp
Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.
[OriTR]+[RFP]
ID: BBa_K1439002
Type: Composite
Length:1448bp
Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
Lysis device induced by L-arabinose
ID: BBa_K1439009
Type: Device
Length:1923bp
Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.
Lysis device induced by aTc
ID: BBa_K1439010
Type: Device
Length:2738bp
Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria.
hEF1a_Cas9-VP16 MammoBlock
ID: BBa_K1179002
Type: Generator
Length: 5141
Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.
TAT-H4
ID:BBa_K1439004
Type: Coding
Length:393
Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.
TAT-PTD
ID:BBa_K1439005
Type: Coding
Length:33
Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.
Histone H4
ID:BBa_K1439006
Type: Coding
Length:312
Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.
CMV promoter
ID:BBa_K1439007
Type: Regulatory
Length:588
Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.
TAT-H4-B0015
ID:BBa_K1439008
Type: Composite
Length:530
Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.
