The Pi NoIR can track near infrared corresponds to wavelengths between 700 and 1000nm. We will use this feature of the camera to track the IFP signal emitted by our CpxR - split IFP1.4 stress responsive cells. The emission of IFP in these wavelengths is especially useful for us, as few things emit auto-fluorescence in the infrared spectrum. This drastically increases the precision of our device as it reduces background noise.

Taking in account all the information above, the main idea driving the way we plan to detect signals through our BioPad detector is to collect the entire light spectrum including near infrared wavelengths and then use a filter to eliminate the visible spectrum.

Light tracking

To track the signal dynamics in the chambers via our detector, we wrote a custom C++ code using OpenCV able to specifically detect the exact position of the signal as well as its nature and intensity. The entire code as well as all the supporting files can be found here: https://github.com/arthurgiroux/igemtracking/. An extract of the main code is given here:

Check out the result of our program here:

To detect the nature of the signal, we used a fairly novel way of processing images and videos. The most common color space used in programming is RGB - pixel colors can be split into three components (Red, Green, and Blue) each taking a value between 0 and 255.

On the other, we used another color space, better adapted for this application as we were especially interested in light intensity and color nuances. We therefore chose the YcrCb color space.

In the YCrCb color space, each pixel is decomposed into three components:

• Y – the luma value (intensity)
• Cr – the red difference
• Cb – the blue difference

This allows us to extract the necessary information for our application.

Lenses

The Raspberry Pi camera that we use has a fixed lens which is not adapted to what we want to do, as we cannot change the focus, the aperture or the zoom.

We searched for a different lens which would allow us more control, and found that the easiest way was to remove the initial Raspberry Pi lens, put a CS mount on it and attach a much bigger lens.

The first thing we did was to unplug the camera module from the PCB. Then, the lens was carefully removed by unscrewing it and the new lens was mounted.

You will then see the camera sensor (CMOS).

The CS mount was screwed on the board and the lens plugged in.

You can see here what the lens sees:

We can clearly see the chambers of the microfluidic chip.