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Team:Groningen/Template/MODULE/project/MBD/specifications&equations
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<table class="table"> | <table class="table"> | ||
<tr> | <tr> | ||
| - | <th> | + | <th>Requirement</th><th>Value</th><th>Note</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>Activation time</td><td>3 h</td><td>Time required for the gel to hydrate after water pockets have been broken to be fully hydrated.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>Therapeutic action time</td><td>?</td><td>After being applied to the wound. Amount of time for reaching the inhibitory concentrations of nisin, DspB and AiiA to pass the bottom membrane and into the wound. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>EMPTY</td><td>EMPTY</td> | + | <td>Number of SA present in the wound </td><td>tbd</td><td>In what quantity does S. aureus need to be detected?</td> |
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Number of PA present in the wound</td><td>tbd</td><td>In what quantity does P. aeruginosa need to be detected?</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Inhibitory nisin concentration</td><td>2-16 ng/µL</td><td>This concentration can be used for both MRSA and VRE. Source: Nisin, alone and combined with peptidoglycan-modulating antibiotics: activity ……. Brumfitt, Salton, Hamilton-Miller.</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Inhibitory AiiA concentration</td><td>tbd</td><td>How much is needed to inhibit growth of PA/SA?</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Inhibitory DspB concentration</td><td>tbd</td><td>How much is needed to inhibit growth of PA/SA?</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Operating temperature</td><td>20-40 °C</td><td>EMPTY</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Lifetime</td><td>3 days</td><td>EMPTY</td> | ||
</tr> | </tr> | ||
Revision as of 21:08, 17 October 2014
Bandage specifications & requirements
Specifications
1. Top layer: Transparent polymethylpentene membrane permeable to gases, flexible.
2. Middle layer: Polyacrylamide hydrogel containing the chemically defined media.
3. Bottom layer: This layer acts as barrier between the wound and the ''L. lactis'' cells. They are inactive since the gel is normally in dehydrated form. This layer consists of a cellulose nitrate membrane of pore size 0.2 micrometers, permeable to protein, being hydrophilic and flexible.
2. Middle layer: Polyacrylamide hydrogel containing the chemically defined media.
3. Bottom layer: This layer acts as barrier between the wound and the ''L. lactis'' cells. They are inactive since the gel is normally in dehydrated form. This layer consists of a cellulose nitrate membrane of pore size 0.2 micrometers, permeable to protein, being hydrophilic and flexible.
Requirements
Most of the requirements were found by placing ourselves in the minds of end-users and derived from interviews with people working at the Martini hospital.
| Requirement | Value | Note |
|---|---|---|
| Activation time | 3 h | Time required for the gel to hydrate after water pockets have been broken to be fully hydrated. |
| Therapeutic action time | ? | After being applied to the wound. Amount of time for reaching the inhibitory concentrations of nisin, DspB and AiiA to pass the bottom membrane and into the wound. |
| Number of SA present in the wound | tbd | In what quantity does S. aureus need to be detected? |
| Number of PA present in the wound | tbd | In what quantity does P. aeruginosa need to be detected? |
| Inhibitory nisin concentration | 2-16 ng/µL | This concentration can be used for both MRSA and VRE. Source: Nisin, alone and combined with peptidoglycan-modulating antibiotics: activity ……. Brumfitt, Salton, Hamilton-Miller. |
| Inhibitory AiiA concentration | tbd | How much is needed to inhibit growth of PA/SA? |
| Inhibitory DspB concentration | tbd | How much is needed to inhibit growth of PA/SA? |
| Operating temperature | 20-40 °C | EMPTY |
| Lifetime | 3 days | EMPTY |
