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Team:Groningen/Template/MODULE/project/MBD/specifications&equations
From 2014.igem.org
Bandage specifications & requirements
Specifications
1. Top layer: Transparent polymethylpentene membrane permeable to gases, flexible.
2. Middle layer: Polyacrylamide hydrogel containing the chemically defined media.
3. Bottom layer: This layer acts as barrier between the wound and the ''L. lactis'' cells. They are inactive since the gel is normally in dehydrated form. This layer consists of a cellulose nitrate membrane of pore size 0.2 micrometers, permeable to protein, being hydrophilic and flexible.
2. Middle layer: Polyacrylamide hydrogel containing the chemically defined media.
3. Bottom layer: This layer acts as barrier between the wound and the ''L. lactis'' cells. They are inactive since the gel is normally in dehydrated form. This layer consists of a cellulose nitrate membrane of pore size 0.2 micrometers, permeable to protein, being hydrophilic and flexible.
Requirements
Most of the requirements were found by placing ourselves in the minds of end-users and derived from interviews with people working at the Martini hospital.
1. Start-up: breaking of the water pockets and hydrating gel/activating L. lactis.
2. Operation: growth of L. lactis, detecting quorum molecules and secreting Nisin, AiiA, DspB.
3. Shut-down: nutrient depletion, cell destruction.
2. Operation: growth of L. lactis, detecting quorum molecules and secreting Nisin, AiiA, DspB.
3. Shut-down: nutrient depletion, cell destruction.
| Requirement | Value | Note |
|---|---|---|
| Activation time | 3 h | Time required for the gel to hydrate after water pockets have been broken to be fully hydrated. |
| Therapeutic action time | 4h | After being applied to the wound. Amount of time for reaching the inhibitory concentrations of nisin, DspB and AiiA to pass the bottom membrane and into the wound. |
| Inhibitory nisin concentration | 2-16 ng/µL | This concentration can be used for both MRSA and VRE. Source: Nisin, alone and combined with peptidoglycan-modulating antibiotics: activity ……. Brumfitt, Salton, Hamilton-Miller. |
| Inhibitory AiiA concentration | 15 ng/µL | How much is needed to inhibit growth of PA/SA? |
| Inhibitory DspB concentration | 15 ng/µL | How much is needed to inhibit growth of PA/SA? |
| Operating temperature | 20-40 °C | |
| Lifetime | 3 days |
Equations
