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Team:British Columbia/Notebook/Protocol
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Revision as of 20:54, 17 October 2014
Protocols
PCR
Instruction for colony PCR and regular PCR
Gel Verification
Make a gel and conduct gel elecrophoresis
BioBrick Restriction Digest
Restriction digest with BioBrick plasmid an inserts
BioBrick Ligation
Ligate Biobrick insert to vector
Making Plates
Make LB plates with antibiotics
Liquid media
Make liquid LB media
Competent cells
Preparation of chemically competent cells
Transformation
Transformation of plasmi DNA into chemically competent cells
CRISPR Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides
(Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation
Electrocompetent Cell Production
Make electrocompetent cells
Make Combinatorial Part Libraries
Make combinatorial part libraries from compatible parts
