BioBrick Restriction Digest

Supplies:

• dsH2O
• 10x buffer (1,2,3,4)
• 100x BSA
• Restriction Enzymes (EcoRI, Xbal, SpeI, PstI)
• DNA

Steps:

1. Make a master mix with everything except the DNA. Keep everything on ice.

*depending on DNA concentration*


2. Pipet aliquots to each sample tube and add DNA.


3. Incubate at 37°C for 2-3h or let digest over night at room temperature.