Team:SCU-China/Safety

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SCU-China/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=SCU-China"style="color:#000000"> Official Team Profile </a></td>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Overview">Overview</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Result">Result</a></li>
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<a href="https://2014.igem.org/Team:SCU-China/Modeling"style="color:#000000"> Modeling</a></td>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Seventh">Presentation at Seventh Senior High School</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/UESTC">Visiting University of Electronic Science and Technology of China (UESTC)</a></li>
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<a href="https://2014.igem.org/Team:SCU-China/Safety"style=" color:#000000"> Safety </a></td>
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<li><a href="https://2014.igem.org/Team:SCU-China/Safety">Safety</a></li>
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<a href="https://2014.igem.org/Team:SCU-China/Attributions"style="color:#000000"> Attributions </a></td>
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<li><a href="https://2014.igem.org/Team:SCU-China/Parts">Parts</a></li>  
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            <li><a href="https://2014.igem.org/Team:SCU-China/Attributions">Attributions</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Biobricks">Notebook of Biobricks</a></li>
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<li><a href="https://2014.igem.org/Team:SCU-China/Transmitter">Notebook of Transmitter</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Effector">Notebook of Effector</a></li>
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                <li class="divider"></li>
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                <li class="dropdown-header">Method</li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Prep">Bacterial Genomic DNA Prep</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Digestion">Digestion</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/GelExtraction">Gel Extraction </a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Linkage">Linkage</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/PCR">PCR</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Plasmid">Plasmid Mini Prep</a></li>
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              </ul>
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<p>For constructing the Biobricks that we want to submit, we set up the PCR protocol and PCR system as bellows:</p><table class="table table-striped"> <caption>PCR system (For 50&#956;L)</caption> <thead><tr><th><p>Materials</p>
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</th><th><p>Volume</p>
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</th>  
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</tr></thead> <tbody><tr><td><p>Taq DNA polymerase</p>
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</td><td><p>0.5&#956;L</p>
</td>
</td>
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</tr><tr><td><p>Template</p>
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</td><td><p>0.5&#956;L</p>
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<tr><td > <h3> Welcome! </h3></td>
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<td > <h3> Timeline</h3></td>
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<td width="45%"  valign="top">
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<br>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<ul>
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<li> <a href=" ">Learn about lab Safety for Today</a></li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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-->
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_2.png|The inside of our lab!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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Image:Example2_Lab_7.png|We decorated this part of our lab
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Image:Example2_Lab_8.png|Whatever else you want
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</tr><tr><td><p>Forward Primer</p>
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</td><td><p>1&#956;L</p>
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</td>
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</tr><tr><td><p>Reverse Primer</p>
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</td><td><p>1&#956;L</p>
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</td>
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</tr><tr><td><p>dNTPs (2.5mM)</p>
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</td><td><p>4&#956;L</p>
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</td>
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</tr><tr><td><p>10x Buffer (Mg2+ free)</p>
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</td><td><p>5&#956;L</p>
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</td>
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</tr><tr><td><p>MgCl2 (25mM)</p>
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</td><td><p>3&#956;L</p>
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</td>
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</tr><tr><td><p>Sterilized diluted water</p>
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</td><td><p>35&#956;L</p>
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</td>
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</tr></tbody>
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</table><p>Note: The content of template should be less than 500ng for the 50&#956;L system. For all of our experiments, the content of template of 0.5&#956;L DNA lower than 500ng.</p>
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<table class="table table-striped"> <caption>PCR protocol</caption><thead><tr><th><p>Temperature</p>
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<td width="45%"  valign="top">  
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</th><th><p>Times</p>
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</th>
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</tr></thead> <tbody><tr><td><p>95&#8451;</p>
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<ul>
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</td><td><p>5min.</p>
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<li> <b>Now :</b> Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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</td><th rowspan="3">30-35 Times</th>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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</tr><tr><td><p>95&#8451;</p>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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</td><td><p>30sec.</p>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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</td>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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</tr><tr><td><p>55-65&#8451;</p>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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</td><td><p>30sec.</p>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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</ul>
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</td>
</td>
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</tr><tr><td><p>72&#8451;</p>
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</td><td><p>1-2min.</p>
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</td>
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</tr><tr><td><p>72&#8451;</p>
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</td><td><p>10min.</p>
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</td>
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</tr></tbody>
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</table><p>Note: for finding the optimal annealing temperature, we have done a series of gradient of annealing temperature first. And as for the elongation time, Taq DNA polymerase could synthesize DNA as the speed of 1000-2000 nucleotides per minute.</p>
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  <p>Sichuan university</p>
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Revision as of 20:40, 17 October 2014

PCR Protocol

For constructing the Biobricks that we want to submit, we set up the PCR protocol and PCR system as bellows:

PCR system (For 50μL)

Materials

Volume

Taq DNA polymerase

0.5μL

Template

0.5μL

Forward Primer

1μL

Reverse Primer

1μL

dNTPs (2.5mM)

4μL

10x Buffer (Mg2+ free)

5μL

MgCl2 (25mM)

3μL

Sterilized diluted water

35μL

Note: The content of template should be less than 500ng for the 50μL system. For all of our experiments, the content of template of 0.5μL DNA lower than 500ng.

PCR protocol

Temperature

Times

95℃

5min.

30-35 Times

95℃

30sec.

55-65℃

30sec.

72℃

1-2min.

72℃

10min.

Note: for finding the optimal annealing temperature, we have done a series of gradient of annealing temperature first. And as for the elongation time, Taq DNA polymerase could synthesize DNA as the speed of 1000-2000 nucleotides per minute.

Sichuan university

Retrieved from "http://2014.igem.org/Team:SCU-China/Safety"