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Team:Evry/Notebook/Transposons/08.27.14
From 2014.igem.org
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| - | < | + | <h4> PCR mutageneses of OriVR6Kgamma </h4> |
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The PCR mutagenes is realized with primers 70 and 71. The template is pNK2 purified, with standard protocol of amplification except that the Tm is 60°C and the time of elongation is of most longer (size of plasmid = 6,4 kb). | The PCR mutagenes is realized with primers 70 and 71. The template is pNK2 purified, with standard protocol of amplification except that the Tm is 60°C and the time of elongation is of most longer (size of plasmid = 6,4 kb). | ||
Latest revision as of 20:17, 17 October 2014
PCR mutageneses of OriVR6Kgamma
The PCR mutagenes is realized with primers 70 and 71. The template is pNK2 purified, with standard protocol of amplification except that the Tm is 60°C and the time of elongation is of most longer (size of plasmid = 6,4 kb).
Aug 27
