Team:Evry/Notebook/Transposons/08.27.14
From 2014.igem.org
![Picture](https://static.igem.org/mediawiki/2013/9/90/EvryBiology.png)
PCR mutageneses of OriVR6Kgamma
The PCR mutagenes is realized with primers 70 and 71. The template is pNK2 purified, with standard protocol of amplification except that the Tm is 60°C and the time of elongation is of most longer (size of plasmid = 6,4 kb).
Aug 27