Team:Evry/Notebook/Transposons/08.27.14

PCR mutageneses of OriVR6Kgamma

The PCR mutagenes is realized with primers 70 and 71. The template is pNK2 purified, with standard protocol of amplification except that the Tm is 60°C and the time of elongation is of most longer (size of plasmid = 6,4 kb).