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Team:Caltech/Project/Data
From 2014.igem.org
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<a href = "https://2014.igem.org/Team:Caltech/Project">Overall Project Summary</a> | <a href = "https://2014.igem.org/Team:Caltech/Project">Overall Project Summary</a> | ||
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| - | < | + | <h3>comX export</h3> |
| + | The export domains team made many attempts at isolating a properly assembled construct (export domain--ligand comX--linker--mNeonGreen) in vector pKS001. However, our colony PCRs were never able to verify a properly assembled construct. Instead, gels with lanes containing colony PCRs of colonies that grew on carbenicillin plates were run and ended up showing bands at roughly 100 base pairs (see <b>Figure 1</b> below). | ||
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| + | These bands at ~100bp suggest that the vector does not contain any insert, since a resealed vector containing no insert should contain around 109bp, assuming that the ends of the linearized backbone do not overlap when recircularizing. | ||
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Revision as of 06:25, 16 July 2014
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Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
comX exportThe export domains team made many attempts at isolating a properly assembled construct (export domain--ligand comX--linker--mNeonGreen) in vector pKS001. However, our colony PCRs were never able to verify a properly assembled construct. Instead, gels with lanes containing colony PCRs of colonies that grew on carbenicillin plates were run and ended up showing bands at roughly 100 base pairs (see Figure 1 below). |
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