"
Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers
From 2014.igem.org
(Difference between revisions)
Patrick 1990 (Talk | contribs) |
|||
| Line 31: | Line 31: | ||
<tr> | <tr> | ||
<td style="text-align: center">10 mM</td> | <td style="text-align: center">10 mM</td> | ||
| - | <td> | + | <td>Imidazole</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 56: | Line 56: | ||
<tr> | <tr> | ||
<td style="text-align: center">20 mM</td> | <td style="text-align: center">20 mM</td> | ||
| - | <td> | + | <td>Imidazole</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 81: | Line 81: | ||
<tr> | <tr> | ||
<td style="text-align: center">250 mM</td> | <td style="text-align: center">250 mM</td> | ||
| - | <td> | + | <td>Imidazole</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 19:41, 17 October 2014
Protocols
Ni NA buffers
Lysis buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 10 mM | Imidazole |
Washing buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 20 mM | Imidazole |
Elution buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 250 mM | Imidazole |
