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Team:UI-Indonesia/Project/Approach and Overview
From 2014.igem.org
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| + | <p>Our team genetically enginereed E.coli, an indigenous gut flora, to specifically detect quorum sensing (QS) molecule from V. cholerae. The mutagenic E.coli is able to detect quorum sensing molecule by expressing CqsS histidine-kinases receptor which is naturally expressed in transmembrane domain of V. cholerae. This receptor will sense Cholerae Autoinducer-1 (CAI-1) which is secreted continously and used as a specific interspecies communication signal (Shao Yi, 2014). CAI-1 molecule act as “find me signal” for mutagenic E.coli when QS molecule binds CqsS receptor and activates motility gene, CheZ, which will enable the E.coli to migrate into the source of infection. It will also activate the killing gene which enables E.coli to produce biofilm enzyme degrading matrix such as: α-amylase, substilisin, and nuclease. After that, our E.coli will secrete peptide 1018, a novel antibiotic peptide indigenous to innate regulator immunity peptide from human cathelicidin, killing V. cholerae. By interconecting the principles of pathogen-directed motility with the dual system degrading and killing biofilm, we significantly develop a novel curative approach or even possibility of short term post-disaster vaccine, by connecting it with slow time based safety module. | ||
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<h2>Approach</h2> | <h2>Approach</h2> | ||
<p>Nowadays, the only way to banish biofilm is by avoiding its excretion on the first place. However, in cases when the biofilm has settled in the digestive tract, avoiding it is no longer possible. Synthetic biology is one of the developing knowledge in our everyday world. Here we proposed the novel strategy to combat biofilm infection by using <span style="color:#1e8380;"><b>‘Genius <i>E.coli</i> (GE)’</b></span>, one will be able to detect quorum sensing signal and destroy biofilm made by pathogenic bacteria. <i>E. coli</i> which is a normal flora in the intestines can be modified to secrete components such as alpha amylase, nuclease, and substilisin that will be able to destroy biofilm made by pathogenic bacteria and novel synthetic peptide called peptide 1018, originated from human immunity defense regulator which is enable target cellular stress responses prevent and eradicate biofilm formation. | <p>Nowadays, the only way to banish biofilm is by avoiding its excretion on the first place. However, in cases when the biofilm has settled in the digestive tract, avoiding it is no longer possible. Synthetic biology is one of the developing knowledge in our everyday world. Here we proposed the novel strategy to combat biofilm infection by using <span style="color:#1e8380;"><b>‘Genius <i>E.coli</i> (GE)’</b></span>, one will be able to detect quorum sensing signal and destroy biofilm made by pathogenic bacteria. <i>E. coli</i> which is a normal flora in the intestines can be modified to secrete components such as alpha amylase, nuclease, and substilisin that will be able to destroy biofilm made by pathogenic bacteria and novel synthetic peptide called peptide 1018, originated from human immunity defense regulator which is enable target cellular stress responses prevent and eradicate biofilm formation. | ||
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Revision as of 19:33, 17 October 2014
Overview
Our team genetically enginereed E.coli, an indigenous gut flora, to specifically detect quorum sensing (QS) molecule from V. cholerae. The mutagenic E.coli is able to detect quorum sensing molecule by expressing CqsS histidine-kinases receptor which is naturally expressed in transmembrane domain of V. cholerae. This receptor will sense Cholerae Autoinducer-1 (CAI-1) which is secreted continously and used as a specific interspecies communication signal (Shao Yi, 2014). CAI-1 molecule act as “find me signal” for mutagenic E.coli when QS molecule binds CqsS receptor and activates motility gene, CheZ, which will enable the E.coli to migrate into the source of infection. It will also activate the killing gene which enables E.coli to produce biofilm enzyme degrading matrix such as: α-amylase, substilisin, and nuclease. After that, our E.coli will secrete peptide 1018, a novel antibiotic peptide indigenous to innate regulator immunity peptide from human cathelicidin, killing V. cholerae. By interconecting the principles of pathogen-directed motility with the dual system degrading and killing biofilm, we significantly develop a novel curative approach or even possibility of short term post-disaster vaccine, by connecting it with slow time based safety module.
