Team:Bielefeld-CeBiTec/Results/Outlook

From 2014.igem.org

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As described in the section about the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/AdhA">alcohol dehydrogenase</a> we could show the successful overexpression of the protein AdhA from <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#L.lactis"><i>Lactococcus lactis</i></a> in <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#E.coli"><i>E. coli</i></a>.
As described in the section about the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/AdhA">alcohol dehydrogenase</a> we could show the successful overexpression of the protein AdhA from <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#L.lactis"><i>Lactococcus lactis</i></a> in <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#E.coli"><i>E. coli</i></a>.
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<br> Further analysis could be an anaerobic cultivation for a complementation analysis of the strain <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs#DeltaAdh">&Delta;adhE748::kan</a>. <a href="#Cong2011">Cong et al., 2011</a> showed that <i>E. coli</i> cannot grow after the loss of its alcohol dehydrogenase.
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<br> Further analysis could be an anaerobic cultivation for a complementation analysis of the strain <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs#DeltaAdh">&Delta;adhE748::kan</a>. <a href="#Trinh2011">Trinh et al., 2011</a> showed that <i>E. coli</i> cannot grow after the loss of its alcohol dehydrogenase.
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   <h6>References</h6>
   <h6>References</h6>
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Pamela P. Peralta-Yahya, Fuzhong Zhang, Stephen B. del Cardayre & Jay D. Keasling, 2012. Microbial engineering for the production of advanced biofuels. In: <a href="http://www.nature.com/nature/journal/v488/n7411/full/nature11478.html" target="_blank">Nature 488</a>, 320–328
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Revision as of 19:32, 17 October 2014


Module III - Isobutanol production

Isobutanol pathway

Alcohol dehydrogenase

As described in the section about the alcohol dehydrogenase we could show the successful overexpression of the protein AdhA from Lactococcus lactis in E. coli.
Further analysis could be an anaerobic cultivation for a complementation analysis of the strain ΔadhE748::kan. Trinh et al., 2011 showed that E. coli cannot grow after the loss of its alcohol dehydrogenase.

References
  • Trinh CT, Li J, Blanch HW, Clark DS., 2011. Redesigning Escherichia coli Metabolism for Anaerobic Production of Isobutanol. In: Appl Environ Microbiol., 77(14): 4894-904

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