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Team:Bielefeld-CeBiTec/Results/Outlook
From 2014.igem.org
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<h6>Alcohol dehydrogenase</h6> | <h6>Alcohol dehydrogenase</h6> | ||
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| - | + | As described in the section about the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/AdhA">alcohol dehydrogenase</a> we could show the successful overexpression of the protein AdhA from <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#L.lactis"><i>Lactococcus lactis</i></a> in <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#E.coli"><i>E. coli</i></a>. | |
| + | <br> Further analysis could be an anaerobic cultivation for a complementation analysis of the strain <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs#DeltaAdh">ΔadhE748::kan</a>. <a href="#Cong2011">Cong et al., 2011</a> showed that <i>E. coli</i> cannot grow after the loss of its alcohol dehydrogenase. | ||
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| + | <h6>References</h6> | ||
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| + | <li id="Pamela2012"> | ||
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| + | Pamela P. Peralta-Yahya, Fuzhong Zhang, Stephen B. del Cardayre & Jay D. Keasling, 2012. Microbial engineering for the production of advanced biofuels. In: <a href="http://www.nature.com/nature/journal/v488/n7411/full/nature11478.html" target="_blank">Nature 488</a>, 320–328 | ||
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| + | <li id="Atsumi2008"> | ||
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| + | Trinh CT, Li J, Blanch HW, Clark DS., 2011. Redesigning Escherichia coli Metabolism for Anaerobic Production of Isobutanol. In: <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147371/">Appl Environ Microbiol.</a>, 77(14): 4894-904 | ||
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Revision as of 19:31, 17 October 2014
Module III - Isobutanol production
Isobutanol pathway
Alcohol dehydrogenase
As described in the section about the alcohol dehydrogenase we could show the successful overexpression of the protein AdhA from Lactococcus lactis in E. coli.
Further analysis could be an anaerobic cultivation for a complementation analysis of the strain ΔadhE748::kan. Cong et al., 2011 showed that E. coli cannot grow after the loss of its alcohol dehydrogenase.
References
-
Pamela P. Peralta-Yahya, Fuzhong Zhang, Stephen B. del Cardayre & Jay D. Keasling, 2012. Microbial engineering for the production of advanced biofuels. In: Nature 488, 320–328
-
Trinh CT, Li J, Blanch HW, Clark DS., 2011. Redesigning Escherichia coli Metabolism for Anaerobic Production of Isobutanol. In: Appl Environ Microbiol., 77(14): 4894-904
