Team:Evry/Notebook/Transposons/10.02.14

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<h1> Golden Gate Is10 </h1>
<h1> Golden Gate Is10 </h1>
The PCR of Is10 kan GFP Is10 didn’t work, there is no amplification.  
The PCR of Is10 kan GFP Is10 didn’t work, there is no amplification.  
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<h1> Golden Gate Universal Transposon Plasmid </h1>
<h1> Golden Gate Universal Transposon Plasmid </h1>
The PCR product to build the Universal Transposon Plasmid is purified, and a Golden Gate is realized with plasmid pSB1C3 (RFC 92).  
The PCR product to build the Universal Transposon Plasmid is purified, and a Golden Gate is realized with plasmid pSB1C3 (RFC 92).  

Latest revision as of 18:54, 17 October 2014

Picture

Migration on the gel of PCR products.
Gel :

Golden Gate Is10

The PCR of Is10 kan GFP Is10 didn’t work, there is no amplification.

Golden Gate Universal Transposon Plasmid

The PCR product to build the Universal Transposon Plasmid is purified, and a Golden Gate is realized with plasmid pSB1C3 (RFC 92).

Oct 02