Team:Evry/Notebook/Transposons/10.02.14
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<h1> Golden Gate Is10 </h1> | <h1> Golden Gate Is10 </h1> | ||
The PCR of Is10 kan GFP Is10 didn’t work, there is no amplification. | The PCR of Is10 kan GFP Is10 didn’t work, there is no amplification. | ||
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<h1> Golden Gate Universal Transposon Plasmid </h1> | <h1> Golden Gate Universal Transposon Plasmid </h1> | ||
The PCR product to build the Universal Transposon Plasmid is purified, and a Golden Gate is realized with plasmid pSB1C3 (RFC 92). | The PCR product to build the Universal Transposon Plasmid is purified, and a Golden Gate is realized with plasmid pSB1C3 (RFC 92). |
Latest revision as of 18:54, 17 October 2014
Migration on the gel of PCR products.
Gel :