Migration on the gel of PCR products.
Gel :

Golden Gate Is10

The PCR of Is10 kan GFP Is10 didn’t work, there is no amplification.

Golden Gate Universal Transposon Plasmid

The PCR product to build the Universal Transposon Plasmid is purified, and a Golden Gate is realized with plasmid pSB1C3 (RFC 92).

Oct 02