<!--TYPO3SEARCH_begin--><div id="c248" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Methanol extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials:&nbsp;</b></p></div><div><p>- Methanol</p></div><div><p>- 37% hydrochloric acid</p></div><div><p>- Centrifuge</p></div><div><p>- Tubes</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>Remove culture media, weigh the remaining pellet, add the same amount of methanol and vortex for 5 minutes. Separate supernatant and pellet and acetify each with about 300µl of 37% hydrochloric acid till the color turns to red.&nbsp;</p></div><div><p>Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.&nbsp;</p></div><div></div></div></div><!--TYPO3SEARCH_end-->