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Team:Hong Kong HKUST/riboregulator/parts
From 2014.igem.org
(Difference between revisions)
| Line 34: | Line 34: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015">BBa_K1379015</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015">BBa_K1379015</a></td> | ||
| - | <td></td> | + | <td>RBS</td> |
<td>RBS(Lock 3C)</td> | <td>RBS(Lock 3C)</td> | ||
<td>12</td> | <td>12</td> | ||
| Line 41: | Line 41: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379018">BBa_K1379018</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379018">BBa_K1379018</a></td> | ||
| - | <td></td> | + | <td>RNA</td> |
<td>HKUST Lock 1</td> | <td>HKUST Lock 1</td> | ||
<td>45</td> | <td>45</td> | ||
| Line 48: | Line 48: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379019">BBa_K1379019</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379019">BBa_K1379019</a></td> | ||
| - | <td></td> | + | <td>RNA</td> |
<td>HKUST Key 1</td> | <td>HKUST Key 1</td> | ||
<td>82</td> | <td>82</td> | ||
| Line 55: | Line 55: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td> | ||
| - | <td></td> | + | <td>Translational Unit</td> |
<td>Lock 1-GFP-B0015</td> | <td>Lock 1-GFP-B0015</td> | ||
<td>903</td> | <td>903</td> | ||
| Line 62: | Line 62: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td> | ||
| - | <td></td> | + | <td>Translational Unit</td> |
<td>Lock 3-GFP-B0015</td> | <td>Lock 3-GFP-B0015</td> | ||
<td>905</td> | <td>905</td> | ||
| Line 69: | Line 69: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td> | ||
| - | <td></td> | + | <td>Translational Unit</td> |
<td>Lock 3C-GFP-B0015</td> | <td>Lock 3C-GFP-B0015</td> | ||
<td>905</td> | <td>905</td> | ||
| Line 76: | Line 76: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td> | ||
| - | <td></td> | + | <td>Translational Unit</td> |
<td>K175031-GFP-B0015</td> | <td>K175031-GFP-B0015</td> | ||
<td>910</td> | <td>910</td> | ||
| Line 83: | Line 83: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td> | ||
| - | <td></td> | + | <td>Translational Unit</td> |
<td>HKUST Lock 1-GFP-B0015</td> | <td>HKUST Lock 1-GFP-B0015</td> | ||
<td>908</td> | <td>908</td> | ||
| Line 91: | Line 91: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td> | ||
| - | <td></td> | + | <td>Translational Unit</td> |
<td>Lock 1-GFP-B0015</td> | <td>Lock 1-GFP-B0015</td> | ||
<td>873</td> | <td>873</td> | ||
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<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td> | ||
| - | <td></td> | + | <td>Translational Unit</td> |
<td>Lock 3C-GFP-B0015</td> | <td>Lock 3C-GFP-B0015</td> | ||
<td>875</td> | <td>875</td> | ||
| Line 106: | Line 106: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td> | ||
| - | <td></td> | + | <td>Composite</td> |
<td>J23102-Lock 1-GFP-B0015</td> | <td>J23102-Lock 1-GFP-B0015</td> | ||
<td>2403</td> | <td>2403</td> | ||
| Line 234: | Line 234: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379021">BBa_K1379021</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379021">BBa_K1379021</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>Key 1-B0015</td> | <td>Key 1-B0015</td> | ||
<td>231</td> | <td>231</td> | ||
| Line 241: | Line 241: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>Key 3-B0015</td> | <td>Key 3-B0015</td> | ||
<td>231</td> | <td>231</td> | ||
| Line 248: | Line 248: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379025">BBa_K1379025</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379025">BBa_K1379025</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>Key 3C-B0015</td> | <td>Key 3C-B0015</td> | ||
<td>231</td> | <td>231</td> | ||
| Line 255: | Line 255: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>K175032-B0015</td> | <td>K175032-B0015</td> | ||
<td>221</td> | <td>221</td> | ||
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<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>HKUST Key 1-B0015</td> | <td>HKUST Key 1-B0015</td> | ||
<td>219</td> | <td>219</td> | ||
| Line 269: | Line 269: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379030">BBa_K1379030</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379030">BBa_K1379030</a></td> | ||
| - | <td></td> | + | <td>Generator</td> |
<td>J23102-Lock 1-GFP-B0015</td> | <td>J23102-Lock 1-GFP-B0015</td> | ||
<td>946</td> | <td>946</td> | ||
| Line 276: | Line 276: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379031">BBa_K1379031</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379031">BBa_K1379031</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>pBad/araC-Key 1</td> | <td>pBad/araC-Key 1</td> | ||
<td>1312</td> | <td>1312</td> | ||
| Line 283: | Line 283: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379032">BBa_K1379032</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379032">BBa_K1379032</a></td> | ||
| - | <td></td> | + | <td>Generator</td> |
<td>J23102-Lock 3-GFP-B0015</td> | <td>J23102-Lock 3-GFP-B0015</td> | ||
<td>948</td> | <td>948</td> | ||
| Line 290: | Line 290: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379033">BBa_K1379033</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379033">BBa_K1379033</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>pBad/araC-Key 3</td> | <td>pBad/araC-Key 3</td> | ||
<td>1312</td> | <td>1312</td> | ||
| Line 297: | Line 297: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379034">BBa_K1379034</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379034">BBa_K1379034</a></td> | ||
| - | <td></td> | + | <td>Generator</td> |
<td>J23102-Lock 3C-GFP-B0015</td> | <td>J23102-Lock 3C-GFP-B0015</td> | ||
<td>948</td> | <td>948</td> | ||
| Line 304: | Line 304: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379035">BBa_K1379035</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379035">BBa_K1379035</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>pBad/araC-Key 3C</td> | <td>pBad/araC-Key 3C</td> | ||
<td>1312</td> | <td>1312</td> | ||
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<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379036">BBa_K1379036</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379036">BBa_K1379036</a></td> | ||
| - | <td></td> | + | <td>Generator</td> |
<td>J23102-K175031-GFP-B0015</td> | <td>J23102-K175031-GFP-B0015</td> | ||
<td>953</td> | <td>953</td> | ||
| Line 318: | Line 318: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379037">BBa_K1379037</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379037">BBa_K1379037</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>pBad/araC-K175032</td> | <td>pBad/araC-K175032</td> | ||
<td>1302</td> | <td>1302</td> | ||
| Line 325: | Line 325: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379038">BBa_K1379038</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379038">BBa_K1379038</a></td> | ||
| - | <td></td> | + | <td>Generator</td> |
<td>J23102-HKUST Key 1-GFP-B0015</td> | <td>J23102-HKUST Key 1-GFP-B0015</td> | ||
<td>951</td> | <td>951</td> | ||
| Line 332: | Line 332: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379039">BBa_K1379039</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379039">BBa_K1379039</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>pBad/araC-HKUST Key 1</td> | <td>pBad/araC-HKUST Key 1</td> | ||
<td>1300</td> | <td>1300</td> | ||
| Line 339: | Line 339: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379040">BBa_K1379040</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379040">BBa_K1379040</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>J23012-Lock 1-GFP-B0015</td> | <td>J23012-Lock 1-GFP-B0015</td> | ||
<td></td> | <td></td> | ||
| Line 346: | Line 346: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379041">BBa_K1379041</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379041">BBa_K1379041</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>J23102-Lock 3-GFP-B0015</td> | <td>J23102-Lock 3-GFP-B0015</td> | ||
<td></td> | <td></td> | ||
| Line 353: | Line 353: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379042">BBa_K1379042</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379042">BBa_K1379042</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>J23102-Lock 3C-GFP-B0015</td> | <td>J23102-Lock 3C-GFP-B0015</td> | ||
<td></td> | <td></td> | ||
| Line 360: | Line 360: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379043">BBa_K1379043</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379043">BBa_K1379043</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>J23102-K175031-GFP-B0015</td> | <td>J23102-K175031-GFP-B0015</td> | ||
<td></td> | <td></td> | ||
| Line 367: | Line 367: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379044">BBa_K1379044</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379044">BBa_K1379044</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>J23102-HKUST Lock 1-GFP-B0015</td> | <td>J23102-HKUST Lock 1-GFP-B0015</td> | ||
<td></td> | <td></td> | ||
| Line 374: | Line 374: | ||
<tr> | <tr> | ||
<td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379049">BBa_K1379049</a></td> | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379049">BBa_K1379049</a></td> | ||
| - | <td></td> | + | <td>Intermediate</td> |
<td>J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015</td> | <td>J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015</td> | ||
<td></td> | <td></td> | ||
Revision as of 18:14, 17 October 2014
Riboregulator Parts
Parts that we designed and submitted
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1379015 | RBS | RBS(Lock 3C) | 12 |
| BBa_K1379018 | RNA | HKUST Lock 1 | 45 |
| BBa_K1379019 | RNA | HKUST Key 1 | 82 |
| BBa_K1379020 | Translational Unit | Lock 1-GFP-B0015 | 903 |
| BBa_K1379022 | Translational Unit | Lock 3-GFP-B0015 | 905 |
| BBa_K1379024 | Translational Unit | Lock 3C-GFP-B0015 | 905 |
| BBa_K1379026 | Translational Unit | K175031-GFP-B0015 | 910 |
| BBa_K1379028 | Translational Unit | HKUST Lock 1-GFP-B0015 | 908 |
| BBa_K1379046 | Translational Unit | Lock 1-GFP-B0015 | 873 |
| BBa_K1379047 | Translational Unit | Lock 3C-GFP-B0015 | 875 |
| BBa_K1379048 | Composite | J23102-Lock 1-GFP-B0015 | 2403 |
Parts that we constructed and submitted
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_I0500 | Inducible pBad/araC promoter | Inducible pBad/araC promoter | 1210 |
| BBa_J01010 | Riboregulator Lock 1 | Biobrick version of Isaacs' riboregulator cis repressed lock, crR12. | 40 |
| BBa_J01080 | Lock3 | Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1. | 40 |
| BBa_J01086 | Key3 | Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1. | 94 |
| BBa_K175031 | Lock for medium RBS (B0032) from the lock/key library TUD09 | This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS. | 47 |
| BBa_K175032 | Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09 | This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031). | 84 |
| BBa_J23080 | B0034 derived RBS to match spacing of J01122 | RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122. | 10 |
Parts that already exist in Parts Registry and are currently under characterization
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_J01008 | Riboregulator Lock 1 | Lock 1 | 94 |
| BBa_J23031 | Lock 3c | lock3c | 42 |
| BBa_J23008 | Key 3c | Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP. | 42 |
Parts that we designed but did not submit
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1379021 | Intermediate | Key 1-B0015 | 231 |
| BBa_K1379023 | Intermediate | Key 3-B0015 | 231 |
| BBa_K1379025 | Intermediate | Key 3C-B0015 | 231 |
| BBa_K1379027 | Intermediate | K175032-B0015 | 221 |
| BBa_K1379029 | Intermediate | HKUST Key 1-B0015 | 219 |
| BBa_K1379030 | Generator | J23102-Lock 1-GFP-B0015 | 946 |
| BBa_K1379031 | Intermediate | pBad/araC-Key 1 | 1312 |
| BBa_K1379032 | Generator | J23102-Lock 3-GFP-B0015 | 948 |
| BBa_K1379033 | Intermediate | pBad/araC-Key 3 | 1312 |
| BBa_K1379034 | Generator | J23102-Lock 3C-GFP-B0015 | 948 |
| BBa_K1379035 | Intermediate | pBad/araC-Key 3C | 1312 |
| BBa_K1379036 | Generator | J23102-K175031-GFP-B0015 | 953 |
| BBa_K1379037 | Intermediate | pBad/araC-K175032 | 1302 |
| BBa_K1379038 | Generator | J23102-HKUST Key 1-GFP-B0015 | 951 |
| BBa_K1379039 | Intermediate | pBad/araC-HKUST Key 1 | 1300 |
| BBa_K1379040 | Intermediate | J23012-Lock 1-GFP-B0015 | |
| BBa_K1379041 | Intermediate | J23102-Lock 3-GFP-B0015 | |
| BBa_K1379042 | Intermediate | J23102-Lock 3C-GFP-B0015 | |
| BBa_K1379043 | Intermediate | J23102-K175031-GFP-B0015 | |
| BBa_K1379044 | Intermediate | J23102-HKUST Lock 1-GFP-B0015 | |
| BBa_K1379049 | Intermediate | J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 |
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