Team:CSU Fort Collins/Notebook/KillSwitch/Sep

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Revision as of 18:09, 17 October 2014

Kill Switch Notes - September

Kill Switch Daily Notes

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July

TRP/KillerRed Assembly

August

The Month of Failed Transformations

September

Troubleshooting and Biobrick Success

SEPTEMBER

September 7

Two ligations completed, one with a 3:2 ration the other a 3:3 ratio of killer red and TRP.


Ligation Diagram

September 9

Ligation of the 3:3 ratio containing the Killer Red Digest and the psB1C3 Digest.

September 23

Digestion of Backbone again labeling the vial C2. Ran gel to see if the ligation was done correctly. It appeared that it was not run correctly; however the new ladder and dye being used did not show up because of its concentration.

September 28

Ligation between KR1 and C2 (backbone digest). Completed transformation via the Cloning a Gene into a Plasmid protocol.

September 30

Completed miniprep of KR Psb1.

OCTOBER

Wednesday, October 8

Sent miniprepped part for sequencing. Submitted KillerRed + Tryptophan BioBrick to iGEM headquarters.

Friday, October 10

Ran gel on miniprep of part to double check accuracy. Gel successful.

Saturday, October 11

Completed an experiment to develop a model for the part and provide proof that it works as expected.



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