Team:Evry/Notebook/Transposons/08.27.14

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Revision as of 17:36, 17 October 2014

Picture

PCR mutageneses of OriVR6Kgamma

The PCR mutagenes is realized with primers 70 and 71. The template is pNK2 purified, with standard protocol of amplification except that the Tm is 60°C and the time of elongation is of most longer (size of plasmid = 6,4 kb).

Aug 27

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