Team:NUDT CHINA/Safety

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<h3>Safety</h3></p><p>
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<h5>Organisms and Parts</h5></p>
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<p><i>Organisms</i></p>
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<p>This year, we used different species of E.coli in our project. Different Genotype provided us different choices when planning our experiment. Based on our project, following 4 species of E.coli was used in our project. All of these species are common and widely used in the field of molecular clone.
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<tr><td width="10%"><strong>Strain</strong></td><td width="10%"><strong>Species name</strong></td><td width="30%"><strong>Genotype</strong></td><td width="10%"><strong>Rish Group</strong></td><td width="10%"><strong>Rish Group Source</strong></td><td width="30%"><strong>Disease risk to humans</strong></td></tr>
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<tr><td>DH5α</td><td><i>Escherichia coli</i></td><td>F-，φ80dlacZΔM15，Δ(lacZYA-argF)U169，deoR，recA1，endA1，hsdR17(rk-，mk+)，phoA，supE44，λ-，thi-1，gyrA96，relA1</td><td>1</td><td>PSDS</td><td>low infectious dose</td></tr>
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<tr><td>Top10</td><td><i>Escherichia coli</i></td><td>F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16, rpsL(StrR), endA1, λ-</td><td>1</td><td>PSDS</td><td>low infectious dose</td></tr>
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<tr><td>BL21（DE3）</td><td><i>Escherichia coli</i></td><td>F-，ompT， hsdS（rBB-mB－），gal， dcm（DE3）</td><td>1</td><td>PSDS</td><td>low infectious dose</td></tr>
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<tr><td>MC1061/P3</td><td><i>Escherichia coli</i></td><td>F-, hsdR, (rk-, mk+) araD139, Δ(araABC-leu)7679, galU, galK, ΔlacX74, rpsL, thi, mcrB, {P3: KanR, AmpR,(am) TetR, (am)}</td><td>1</td><td>PSDS</td><td>low infectious dose</td></tr>
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<p><i>Parts</i></p>
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<p>Our parts are all assembled with materials from the distribution or synthesized based on known sequence.
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For more detaile, please read the ’’part’’ section of our wiki.</p>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA/Team">Team</a> </li>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:NUDT_CHINA/Attributions">Attributions</a> </li>
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<a href="https://2014.igem.org/wiki/index.php?title=Team:NUDT_CHINA/Safety&action=edit"> Click here to edit this page!</a>
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<a href="https://2014.igem.org/Special:Upload">Click here to upload! </a>
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Revision as of 17:31, 17 October 2014


Safety

Organisms and Parts

Organisms

This year, we used different species of E.coli in our project. Different Genotype provided us different choices when planning our experiment. Based on our project, following 4 species of E.coli was used in our project. All of these species are common and widely used in the field of molecular clone.

StrainSpecies nameGenotypeRish GroupRish Group SourceDisease risk to humans
DH5αEscherichia coliF-，φ80dlacZΔM15，Δ(lacZYA-argF)U169，deoR，recA1，endA1，hsdR17(rk-，mk+)，phoA，supE44，λ-，thi-1，gyrA96，relA11PSDSlow infectious dose
Top10Escherichia coliF-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16, rpsL(StrR), endA1, λ-1PSDSlow infectious dose
BL21（DE3）Escherichia coliF-，ompT， hsdS（rBB-mB－），gal， dcm（DE3）1PSDSlow infectious dose
MC1061/P3Escherichia coliF-, hsdR, (rk-, mk+) araD139, Δ(araABC-leu)7679, galU, galK, ΔlacX74, rpsL, thi, mcrB, {P3: KanR, AmpR,(am) TetR, (am)}1PSDSlow infectious dose

Parts

Our parts are all assembled with materials from the distribution or synthesized based on known sequence. For more detaile, please read the ’’part’’ section of our wiki.

Click here to edit this page! Click here to upload!

WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Welcome!

Timeline

Visit the Safety Hub to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.


Edit this page!

Please use this page to write about anything related to safety in your project.

Your Lab

Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space!

  • Now : Read the Safety Hub and learn about safety in iGEM. Ask questions by emailing safety at igem DOT org .
  • Now - Jamboree: Complete Check-Ins and receive approval before acquiring and using certain materials in your lab
  • Now - Wiki Freeze: Edit this Safety page to tell us about what you're doing
  • June 9: Submit the About Our Lab form.
  • Let us know by June 25 if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.
  • June 30: Submit the Preliminary Version of the Safety Form.
  • Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).
  • September 1: Submit the Final Version of the Safety Form.
  • October: Wiki freeze (exact date to be determined)
  • October 30 - November 3: GIANT JAMBOREE!

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