Team:CSU Fort Collins/Notebook/HVP/Jun
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<h4>Monday, June 9 - Monday, June 16</h4> | <h4>Monday, June 9 - Monday, June 16</h4> | ||
- | Researched the mevalonate pathway in E. coli and sequenced the genes to be inserted into puc19. Designed primers as specified in the <a href='/Team:CSU_Fort_Collins/Notebook/Protocols=Gibson'>Gibson Assembly Protocol</a> for transformation of the mevalonate pathway from yeast into <u>E. coli</u>. | + | Researched the mevalonate pathway in <u>E. coli</u> and sequenced the genes to be inserted into puc19. Designed primers as specified in the <a href='/Team:CSU_Fort_Collins/Notebook/Protocols=Gibson'>Gibson Assembly Protocol</a> for transformation of the mevalonate pathway from yeast into <u>E. coli</u>. |
<h4>Thursday, June 19</h4> | <h4>Thursday, June 19</h4> | ||
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<h4>Wednesday, June 25</h4> | <h4>Wednesday, June 25</h4> | ||
- | Took a small sample of E. coli with puc19 from a previous colony and suspended in LB Broth. Shook at 225 rpm in incubator at 37° C overnight to culture. | + | Took a small sample of <u>E. coli</u> with puc19 from a previous colony and suspended in LB Broth. Shook at 225 rpm in incubator at 37° C overnight to culture. |
<h4>Thursday, June 26</h4> | <h4>Thursday, June 26</h4> | ||
- | Completed a miniprep of E. coli with puc19 from the overnight culture. Followed <a href='/Team:CSU_Fort_Collins/Notebook/Protocol/Miniprep'>plasmid miniprep protocol</a> as given in the Informatics (Life Technologies) kit. | + | Completed a miniprep of <u>E. coli</u> with puc19 from the overnight culture. Followed <a href='/Team:CSU_Fort_Collins/Notebook/Protocol/Miniprep'>plasmid miniprep protocol</a> as given in the Informatics (Life Technologies) kit. |
<h4>Friday, June 27</h4> | <h4>Friday, June 27</h4> |
Revision as of 17:24, 17 October 2014
High-Value Product Daily Notes
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Jun
pMBI Assembly
July
pMBI and pMevT Assembly
August
Troubleshoot Everything!
September
Plan B