Reformed Plasmid Result
We designed four reformed plasmids, pBluescript II KS(+) ScaI deletion, pBluescript II KS(+) EcoRV deletion, pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy. And we test their co-transformation ability and get the result picture. All of these plasmid has Amp antibiotic resistance.
We co-transformed pRSFDuet-1 and two plasmids, pBluescript II KS(+) ScaI deletion & pBluescript II KS(+) EcoRV deletion, respectively. So the plate has two antibiotic resistance, Kan and Amp. We culture two different strains on the plate and get the result picture.
![](https://static.igem.org/mediawiki/2014/5/5f/123123123.jpg)
Moreover, we verify our pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy part through lacl & blue-white spot screening.
![](https://static.igem.org/mediawiki/2014/c/c6/212121.jpg)
So we experimentally validate that our reformed plasmids perform as expected.