Team:SJTU-BioX-Shanghai/Results

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Revision as of 16:51, 17 October 2014

Reformed Plasmid Result

We designed four reformed plasmids, pBluescript II KS(+) ScaI deletion, pBluescript II KS(+) EcoRV deletion, pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy. And we test their co-transformation ability and get the result picture. All of these plasmid has Amp antibiotic resistance.
We co-transformed pRSFDuet-1 and two plasmids, pBluescript II KS(+) ScaI deletion & pBluescript II KS(+) EcoRV deletion, respectively. So the plate has two antibiotic resistance, Kan and Amp. We culture two different strains on the plate and get the result picture.

Figure 2.1.1 Two plates of Co-transfected bacteria (pBluescript II KS(+) ScaI deletion & pBluescript II KS(+) EcoRV deletion) Moreover, we verify our pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy part through lacl & blue-white spot screening.

Figure 2.1.2 Two plates of lacl & blue-white spot screening So we experimentally validate that our reformed plasmids perform as expected.

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 <p>We designed four reformed plasmids, pBluescript II KS(+) ScaI deletion, pBluescript II KS(+) EcoRV deletion, pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy.
 And we test their co-transformation ability and get the result picture. All of these plasmid has Amp antibiotic resistance. <br>
-We co-transformed pRSFDuet-1 and these four plasmids, respectively. So the plate has two antibiotic resistance, Kan and Amp. We culture 4 different strains on the plate and get the result picture.
+We co-transformed pRSFDuet-1 and two plasmids, pBluescript II KS(+) ScaI deletion & pBluescript II KS(+) EcoRV deletion, respectively. So the plate has two antibiotic resistance, Kan and Amp. We culture two different strains on the plate and get the result picture.
-<center><img src="" width=600px></img></center>
+<center><img src="https://static.igem.org/mediawiki/2014/5/5f/123123123.jpg" width=600px></img></center>
-Figure 2.1.1 Four plates of Co-transfected bacteria
+Figure 2.1.1 Two plates of Co-transfected bacteria (pBluescript II KS(+) ScaI deletion & pBluescript II KS(+) EcoRV deletion)
-So we experimentally validate that our reformed plasmids perform as expected.
+Moreover, we verify our pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy part through lacl & blue-white spot screening.
+<center><img src="https://static.igem.org/mediawiki/2014/c/c6/212121.jpg" width=600px></img></center>
+Figure 2.1.2 Two plates of lacl & blue-white spot screening
+So we experimentally validate that our reformed plasmids perform as expected.
 </p>
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