Team:Caltech/Project/Methods and Methods
From 2014.igem.org
(Difference between revisions)
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</ul> | </ul> | ||
<b>Thermal Cycler Protocol</b> | <b>Thermal Cycler Protocol</b> | ||
- | <ul><li></li> | + | <ul><li> 98C for 30 seconds </li> |
+ | <li> 98C for 10 seconds </li> | ||
+ | <li> 53C for 30 seconds </li> | ||
+ | <li> 72C for 15<i>x</i> seconds, where <i>x</i> is the expected length of longest PCR product (in kilobases) </li> | ||
+ | <li> Repeat above steps 29 more times </li> | ||
+ | <li> 72C for 10 minutes </li> | ||
+ | <li> Hold at 4C </li> | ||
</ul> | </ul> | ||
Revision as of 21:14, 14 July 2014
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Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
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