"
Team:TU Darmstadt/Notebook/Materials/Buffers
From 2014.igem.org
(Difference between revisions)
BastianW (Talk | contribs)
(Created page with "{{:Team:TU_Darmstadt/Template}} <html> <div id="contentWrap" class="container_24"> <div id="breadcrumbs" class="grid_24"> <p>Sie sind hier: <a href="http://20...")
Newer edit →
(Created page with "{{:Team:TU_Darmstadt/Template}} <html> <div id="contentWrap" class="container_24"> <div id="breadcrumbs" class="grid_24"> <p>Sie sind hier: <a href="http://20...")
Newer edit →
Revision as of 16:16, 17 October 2014
Buffers
Tris (1M, pH 7,5)
- 60,5g Tris base
- adjust pH to 7,5 using 5M HCl
- add ddH2O up to 500 mL of volume
1x PBS
- 8.18 g NaCl (140 mM)
- 0.2 g KCl (2.7 mM)
- 1.77 g Na2HPO4 (10 mM)
- 0.24 g KH2PO4 (1.8 mM)
- add ddH2O up to 1 L of volume
- adjust to pH 7.4
TE buffer
- 10 mM Tris, bring to pH 8.0 with HCl
- 1 mM EDTA (Ethylenediaminetetraacetic acid)
50x TAE
- 242 g Tris base in water
- add 57.1 mL glacial acetic acid
- 100 mL of 500 mM EDTA (pH 8.0) solution
- add ddH2O up to 1 L of volume
