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Team:TU Darmstadt/Notebook/Methods/Agarose gel electrophoresis
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Revision as of 16:01, 17 October 2014
Agarose gel electrophoresis
Short explanation:
Agarose gel electrophoresis is the most common used method to separate nucleic acids. Due to their negativ charge DNA and RNA molecules can be moved through an agarose gel by an electric field (electrophoresis). Longer molecules move slower through the agarose matrix while short move faster and migrate further.
Procedure:
In common we used 1% agarose gel.
1. The hot and liquid agarose gel is mixed with 4 µl NANCY for 50 ml of agarosegel (Shake in a bottle)
2. Fill everything in a chamber and let it cool down (do not forget the well combs)
3. Take 1xTAE-Buffer for the run
4. In the first ten minutes run the gel with 80 V and afterwards select 120V
