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Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers
From 2014.igem.org
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<td style="text-align: center">50 mM</td> | <td style="text-align: center">50 mM</td> | ||
| - | <td> | + | <td>NaH<sub>2</sub>PO<sub>4</sub></td> |
</tr> | </tr> | ||
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<td style="text-align: center">50 mM</td> | <td style="text-align: center">50 mM</td> | ||
| - | <td> | + | <td>NaH<sub>2</sub>PO<sub>4</sub></td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td style="text-align: center">50 mM</td> | <td style="text-align: center">50 mM</td> | ||
| - | <td> | + | <td>NaH<sub>2</sub>PO<sub>4</sub></td> |
</tr> | </tr> | ||
Revision as of 15:33, 17 October 2014
Protocols
Ni NA buffers
Lysis buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 10 mM | Immidazol |
Washing buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 20 mM | Immidazol |
Elution buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 250 mM | Immidazol |
