Team:Tuebingen/Notebook/Protocols/OligoAnnealing

From 2014.igem.org

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<h1>Protocols</h1>
<h3>Oligo Annealing</h3>
<h3>Oligo Annealing</h3>

Latest revision as of 15:23, 17 October 2014


Protocols

Oligo Annealing

Reagents

96 µL TE-buffer + 56 mM NaCl
2 µL Primer forward
2 µL Primer reverse

 

Procedure

  1. Dilute the primers in buffer.
  2. Heat the solution to 98 °C and decrease temperature slowly to 25 °C.
  3. Use for ligation.

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