Team:Tuebingen/Notebook/Protocols/OligoAnnealing

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Revision as of 15:16, 17 October 2014


Oligo Annealing

Reagents

96 µL TE-buffer + 56 mM NaCl
2 µL Primer forward
2 µL Primer reverse

Procedure

  1. Dilute the primers in buffer.
  2. Heat the solution to 98 °C and decrease temperature slowly to 25 °C.
  3. Use for ligation.

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